Programme

11u00-13u00: Online introduction to get to know the lecturers, other participants and practicalities of the BIP. The expectations for the literature review will be explained during this session.

• 9:00-9:15 - Welcome with coffee 
  
• 9:15-9:30 - Opening session 
• 9:30-11:00 - Ms Kristel Sniegowski, KU Leuven (BE), on ‘Basic molecular techniques to identify wild yeast isolates and the search for POF genes' 
• 11:00-11:15 - Break 
• 11:15-12:45 - Ms Vesna Jerkovic/Charlotte Saussez/Aurélie Semoulin, HELHa (BE), on ‘Bacterial transformation: theorical and practical aspects'
• 12:45-13:45 - Lunch break  
• 13:45-15:00 - Ms Paulien Leemans, UAntwerp (BE) – ‘Gene regulation in prokaryotes’
• 15:00-15:15 - Break 
• 15:15 -16:30 - Ms Iris Cornet, UAntwerp (BE)– ‘Process optimization in industrial biotechnology using recombinant microorganisms.'
• 16:30-17:30 - Break 
• 17:30-19:00 - Guided city tour Antwerp 
• 19:00-21:00 - Welcome’s drink @Stadscampus

• 8:30-12:30:
  • Workshop I - Ms Julia Weiss, UPCT (ES), on ‘Binary plasmid isolation and analysis‘- laboratory 
  • Workshop II - Ms Kristel Sniegowski, KU Leuven (BE), on ‘Detecting POF genes in yeast isolates with PCR and gel electrophoresis‘- laboratory 
  • Workshop III – Mr Boris Gilis, University of Antwerp, on ‘Production of microbial oil in a 25L scale bioreactor’ part 1: reactor-set up 
• 12:30-14:00 - Lunch break 
• 14:00-18:00:      
  • Workshop I - Ms Vesna Jerkovic/Charlotte Saussez/Aurélie Semoulin, HELHa (BE) on ‘Bacterial transformation‘- laboratory 
  • Workshop II - Ms Kristel Sniegowski, KU Leuven (BE), on ’ Evaluating the quality of a Sanger sequence, database search with sequences for identifying Fungi’ - informatic lab 
  • Workshop III - M Rita Pacheco -  ‘Inhibition of Acetylcholinesterase activity- Dose- Response relationships‘ – laboratory 

• 9:00-10:30 - Rita Pacheco, ISEL (PT) – “Small molecule drugs- Design and Drug Discovery“ 
  
• 10:30-11:00 - Lecture (TBD) 
• 11:00-11:15 - Break 
• 11:15 -12:45 - Mr Vuk Savković, Hochschule Merseburg (DE), on ‘Directed evolution and rational design as powerful tools in protein engineering’  
• 12:45-14:00 - Lunch break  
• 14:15-16:15 - Company visit: Antwerp Brewing Company  

• 8:30-12:30
  • Workshop I - Marcos Egea Gutiérrez-Cortines UPCT (ES), on ‘Usage of publicly available tools: sequence data bases and construct design for CRISP/Cas’  – informatic lab 
    
  • Workshop II - Ms Paulien Leemans/Iris Cornet, UAntwerp on ‘Investigation of heat shock effects on the gene expression in E. coli – Part 1: RNA extraction (RNA integrity and purity) and reversed transcription‘– laboratory 
  • Workshop III – Mr Boris Gilis, University of Antwerp, on ‘Production of microbial oil in a 25L scale bioreactor’ part 2: analysis 
• 12:30-14:00 - Lunch break 
• 14:00-18:00 
  • Workshop I – Mr Boris Gilis, University of Antwerp, on ‘Production of microbial oil in a 25L scale bioreactor’ part 2: analysis'
  • Workshop II - Ms Paulien Leemans/Iris Cornet, UAntwerp on ‘Investigation of heat shock effects on the gene expression in E. coli – Part 2: Bioinformatical analysis of RT-qPCR data.'
  • Workshop III - Mr Vuk Savković , Hochschule Merseburg (DE), on‘Searching for the most efficient enzyme variant (known so far) for a particular biochemical transformation’ 

• 9:00 -10:30 - Ms Julia Weiss, UPCT (ES), on ‘Plant genetic engineering‘ 
  
• 10:30-11:15 - From oil to acid – Boris Gilis (PhD student, University of Antwerp) 
• 11:15-11:30 - Break 
• 11:30-13:00 - Mr Marcos Egea Gutiérrez-Cortines and Ms Julia Weiss, UPCT (ES), on  ‘Molecular pharming strategies‘ (TBD) 
• 13:00-14:00 - Break 
• 14:00 - Group work (optional)