Flow cytometry is a method that is used to examine and determine the expression of intracellular molecules and the cell surface and to define and characterize distinct cell types. It is also used in determining the cell volume and cell size and to evaluate the purity of subpopulations that are isolated. This allows the multi-parameter evaluation of single cells at about the same time. Flow cytometry is used in measuring the intensity of fluorescence which is produced due to fluorescently labeled antibodies that help to identify proteins or ligands that bind to associated cells.
