The BD FACSLyric is a new-generation high-performance flow cytometer.
The FACSLyric is a 3-laser flow cytometer that is configured with 12 independent photomultipliers. It is a high-performance, highly sensitive flow cytometer with exceptional resolution, with up to 35.000 events/sec.
Upon request, we provide a one-hour training session for the FACSLyric. Having received this training, the user can independently operate the flow cytometer and use the software FACSuite. Reservations for the FACSLyric can be made through our online calendar. Facility staff is available to go over experimental design, discussion of data analysis, or any other questions users may have.
LaserParameterFilterCommon dyes/fluorochromes*
| 405nm | E | 448/45 | BV421, Alexa Fluor 405, V450, Pacific Blue, VioBlue, Live/Dead Violet |
| D | 528/45 | BV510, Alexa Fluor 430, V500, Amcyan, VioGreen, Live/Dead Aqua | |
| C | 606/36 | BV605, Qdot 605 | |
| B | 715/50 | BV711, Qdot 705, BV750 | |
| A | 755 LP | BV750, BV785, BV786, Qdot 800 | |
| 488nm | D | 527/32 | FITC, Alexa Fluor 488, BB515, Live/Dead Green |
| C | 586/42 | PE, Spark Blue 585, PI | |
| B | 700/54 | PerCP, PerCP-Cy5.5, PE-Cy5, BB700, 7-AAD | |
| A | 786/46 | PE-Cy7, PE-Vio770 | |
| 633nm | C | 660/20 | APC, Alexa Fluor 647, Live/Dead Far Red |
| B | 720/30 | APC-R700 | |
| A | 783/56 | APC-Cy7, APC-H7, APC-Alexa Fluor 750, APC-Vio770, Live/Dead Near-IR |
* This is a list of fluorochromes commonly used on the FACSLyric. The list is not exhaustive, if you would like to use a different fluorochrome, please contact the facility.
