FACSAria II

The FACSAria II, developed by Becton Dickinson, is a benchtop high-speed flow cytometric cell sorter. It has a fixed-alignment cuvette flow cell providing superior fluorescence sensitivity compared to other flow cell types. The optical system is configured with a 488 nm blue solid state laser, a 635 nm red laser, a 405 nm violet laser, and detectors for two different scatter signals in combination with up to 10 fluorescent channels.

High-speed multicolor sorting is possible with digital acquisition rates of up to 20 000 events / second. Cells can be sorted into 5 or 15 mL tubes or into various well-plates as single or multiple cells per well, as well as onto microscope slides for downstream analysis. In addition, our sorter is capable of simultaneous 4-way sorting. The FACSAria II is equipped with an Aerosol Management System, providing an additional level of safety for the operator.

Besides using cell sorting for our own research goals, we are dedicated to the advancement of scientific research within the University of Antwerp and beyond. Upon request we provide a training session for the FACSAria II. Having received this training, the user is able to independently operate the sorter and use the software. Reservations for the FACSAria II can be made through our online calendar. The FACSAria II has dedicated operators who can assist in experimental design, data acquisition and data analysis.

LaserParameterFilterCommon dyes/fluorochromes*

* This is a list of fluorochromes commonly used on the FACSAria II. The list is not exhaustive, if you would like to use a different fluorochrome, please contact the facility. Check the fluorochrome guide.