Equipment and Techniques
Equipment:
- Flow cytometers; BD FACSymphonyTM A1, BD Facs CAliburTM and BD Facs CantoTM II (BD Biosciences)
- Cell culture facility
- Phadia 250 (Thermo Fisher)
- Lux Scan 10K-A microarray scanner (Capital Bio)
- Äkta start protein purification system (GE Healthcare Life Sciences)
- Alpha 1-4 LD plus freeze dryer (Christ)
- SavantTM SPD 111 SpeedVacTM concentrator (Thermo Scientific)
- BX43 upright microscope with U-GAN for Gout diagnostic (Olympus)
- MultiscanTM microplate photometer (Thermo Scientific)
- CytospinTM 4 cytocentrifuge (Thermo Scientific)
- MoxiTM Z automated cellcounter (Orflo)
Techniques:
- Basophil Activation Test (BAT)
- Direct and passive Mast Cell Activation Test (dMAT and pMAT)
- CD34 derived Mast Cell cultures
- Westernblot
- ELISA
- Cytometric Bead Array (CBA)
- Immunofluorescence and live cell immaging