Researchers of the Medical Biochemistry group develop purification methods for the enzymes they study, from natural sources as well as recombinant organisms. They develop assays that can be used to assess activity in complex biological samples and for screening and counterscreening of lead compounds during drug development.
We are open for collaborations with academic groups and pharmaceutical and biotech companies.
Enzymes currently available for drug screening
- Human dipeptidyl peptidase 4 (seminal plasma) – DPP4
- Human dipeptidyl peptidase 2 (seminal plasma) – DPP2
- Bovine dipeptidyl peptidase 9 (testes) – DPP9
- Human prolyl oligopeptidase (recombinant, expressed in E. coli) – hPREP
- Porcine prolyl oligopeptidase (recombinant, expressed in E. coli) – pPREP
- Human carboxypeptidase U (plasma) – proCPU
- Human carboxypeptidase M (soluble form, recombinant expressed in P. pastoris) – CPM
- Human fibroblast activation protein (recombinant, expressed in insect cells) - hFAP
Routine assays for drug screening and/or activity measurements in complex biological samples
- Proline specific dipeptidyl peptidase activity
- Selective assays for DPP4, DPP2, DPP8/DPP9 activity
- Prolylcarboxypeptidase activity (PRCP)
- Prolyl oligopeptidase (endopeptidase) activity
- Fibroblast activating protein (FAP) activity
- Aminopeptidase P (AAP) activity
- Basic carboxypeptidase activity (CPU, CPN, CPM, CPB)
- Selective aCPU activity
- Clot lysis assay in pooled human plasma
- In vitro α-synuclein aggregation assay
Assays on request using commercial enzymes
- Trypsin and chymotrypsin
- Subtilysin
- Thrombin
- Plasmin
- Factor Xa
- Factor XIa
- Factor XIIa
- Factor VIIa
- Urokinase (uPA)
- Tissue plasminogen activator (tPA)
- Plasma kallikrein
- Plasma elastase
- Furin
- MMP-1
- MMP-2
- Proteasome activity (chymotrypsin- and trypsin-like activity)
- Triosephosphate isomerase
- Glyceraldehyde-3-phosphate dehydrogenase
- Hexokinase and glucokinase
- Other enzymes from the glycolytic and glyceroneogenetic pathways
- Thiolase
- Horseradish peroxidase, chloroperoxidase, myeloperoxidase
- Glucose oxidase
- Catalase
- Xylose (Glucose) isomerase
- α-amylase
- Gluco-amylase
- Maltase
- Angiotensin converting enzyme (ACE)
- Adenosine deaminase (ADA)