Research Annemie Van Der Linden

Abstract

Huntington's disease (HD) is a dominantly inherited disorder characterized by a progressive neurodegeneration of the striatum that also involves other regions, primarily the cerebral cortex. Patients display progressive motor, cognitive, and psychiatric impairment. Symptoms usually start at midlife. The mutation responsible for this fatal disease is an abnormally expanded and unstable CAG repeat within the coding region of the gene encoding huntingtin. The pathogenic mechanisms by which mutant huntingtin cause neuronal dysfunction and cell death remain uncertain (Menalled, 2005). The mechanism underlying HD-related suppression of inhibition has been shown to include tonic activity of metabotropic glutamate receptor subtype 5 (mGluR5) as a pathophysiological hallmark (Dvorzhak, Semtner, Faber, & Grantyn, 2013) and inhibition of glutamate neurotransmission via specific interaction with mGluRs might be interesting for both inhibition of disease progression as well as early symptomatic treatment (Scheifer et al., 2004). With the objective to elucidate the role of glutamatergic pathways using small animal PET imaging, this study aims to use several PET imaging agents as tracers in a knock-in model of Huntington's disease.

Researcher(s)

• Promoter: Staelens Steven
• Co-promoter: Bertoglio Daniele
• Co-promoter: Van Der Linden Annemie
• Co-promoter: Verhaeghe Jeroen
• Co-promoter: Verhoye Marleen

Research team(s)

• Molecular Imaging and Radiology (MIRA)

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Van Der Linden Annemie
• Promoter: Verhoye Marleen

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

Upgrade of the hardware of existing equipment (9.4T MRI system from Bruker) to perform state of the art MRI investigations in the brain of small animals such as mice, rats and birds. This hardware upgrade will enable implementation of all new Bruker software packages.

Researcher(s)

• Promoter: Van Der Linden Annemie
• Co-promoter: Keliris Georgios A.
• Co-promoter: Ponsaerts Peter
• Co-promoter: Sijbers Jan
• Co-promoter: Staelens Steven
• Co-promoter: Verhoye Marleen

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

Cultural transmission of vocal behaviours such as human speech or bird song, are greatly influenced by how adults interact with each other and with their young. Even though these behavioural observations are well established, surprisingly, the neurobiological mechanisms via which social enhancement potentiates learning are still poorly understood. Recently, we discovered that future song learning accuracy can be predicted very early in the song learning process based on the structural properties of the auditory areas of the zebra finch brain. Building further on this recent discovery, we aim to (1) identify the neurobiological basis of this prediction; (2) uncover the functional neural circuit that selectively responds to social factors inherent to song learning; and (3) unravel the functional and structural connectivity between the prediction site and remote brain areas. To reach these aims, we will use advanced magnetic resonance imaging (MRI) tools that enable to repeatedly quantify the structural architecture and connectivity of the zebra finch brain along the process of vocal learning. We will validate these insights by advanced histology. Moreover, this will be the first study to employ awake functional MRI in juvenile zebra finches to repeatedly probe brain activation patterns in response to specific stimuli presented by a video. To establish brain-behaviour relationship, we will evaluate the MRI outcome relative to several behavioural measures in the same bird.

Researcher(s)

• Promoter: Verhoye Marleen
• Co-promoter: Van Der Linden Annemie
• Fellow: Bowman Christien

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

The current therapies of Alzheimer's disease (AD) are insufficient and novel treatments are necessary. Cholinergic and noradrenergic neurotransmitter systems are involved in memory and mood modulation. An add-on effect of increased noradrenergic signalling in addition to the standard therapy of increased cholinergic signalling has been proposed for AD patients. However, the interaction between the two systems is not well understood. In this proposal, we will evaluate the effects of activating via DREADDs 1) cholinergic neurons in medial septum, which project to hippocampus, 2) noradrenergic neurons in locus coeruleus, which project to medial septum and hippocampus, and 3) cholinergic and noradrenergic neurons. We will evaluate the effect of these different modulations on behaviour (memory and mood) and different brain network properties in a promising AD rat model and in healthy rats. We will look at functional connectivity in the brain, oscillations in local field potentials in hippocampus (which reflect local hippocampal network properties), and whole-brain activity state related to sharp-wave ripples, a neuronal event that occurs within hippocampus and that is associated with memory. Finally, we will assess whether early deficits in functional connectivity and cerebral blood flow can predict long-term behavioural outcome in (untreated) AD rats and whether deficits in these parameters can predict the responsiveness to the treatment (one of the three possible modulations).

Researcher(s)

• Promoter: Keliris Georgios A.
• Co-promoter: Van Der Linden Annemie
• Fellow: Missault Stephan

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

Light is an important environmental factor driving many functions in animal physiology. There are two systems for detecting light in animals i) The classical visual system for image formation (IF) and ii) The Non-image-forming (NIF) visual circuit. NIF in mammals is mediated by melanopsin containing intrinsically photoreceptive retinal ganglionic cells and innervates brain regions regulating sleep, circadian functions, cognition etc. We hypothesize that the NIF circuit mediates the light's influence in neuroplasticity of seasonal songbirds. To test this, we use European starling's which shows extensive neuroplasticity in response to seasons, in vivo MRI and molecular biology techniques.

Researcher(s)

• Promoter: Van Der Linden Annemie
• Co-promoter: Keliris Georgios A.
• Fellow: Majumdar Gaurav

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

During the last decades, the achievement of a better and improved quality of life has resulted in increased life expectancy. This is mainly due to progress in translational research and development of new therapeutic approaches. The downside is that age is one of the major risk factors for dementias and neurodegenerative disorders such as Alzheimer's disease (AD), characterized by a marked decline of cognitive functions (e.g. short- and long-term memory loss) and dysregulation of higher cortical functions (e.g. impaired judgement and thinking). The pathological condition of these diseases is disabling enough to compromise the activity of everyday life. Lengthening the life span has little value if the quality of life cannot be ensured. Unfortunately, the pathogenesis of AD is still far from being understood and this could be the reason why none of the currently available pharmacological therapies for this disease are satisfactory. Current treatments are purely symptomatic and do not act on the onset and progression of the pathology. It is well known that Basal Forebrain (BF) cholinergic neurons are prone to degeneration during aging as well as in dementias like AD. Furthermore, "the cholinergic hypothesis of geriatric cognitive dysfunction" is also supported by the significant correlation between the level of cholinergic depletion and the degree of cognitive deficits. Acetylcholine is a neuromodulator broadly investigated for its role in learning and memory, but it is not the only player in AD. In fact, in the BF, intermingled with cholinergic neurons, there are also two non-cholinergic neuronal types: GABAergic and glutamatergic neurons. It has been discovered that dysfunctions at the level of glutamatergic and GABAergic systems are involved as well in AD. Until recently, neuroscientists have limited the research of AD to the study of a single neuronal type (mainly BF cholinergic neurons), overlooking the possible role of non-cholinergic neuronal populations (GABAergic and glutamatergic). However, it is of the utmost importance to uncover the interaction between BF cholinergic and non-cholinergic neurons to develop novel strategies for the treatment of AD. The proposed research project aims to investigate the interaction between the three distinct BF populations and to elucidate how the BF cholinergic neuronal activity influences the other two BF neuronal types both in healthy and in pathological conditions. To date, it is still far from being understood how the neural state of the cholinergic neurons influences the GABAergic and glutamatergic neurons in the BF and how these, in turn, adjust cholinergic neuromodulation. We suggest to study the activity of BF neuronal populations and their interactions during spontaneous activity and determine the relationship of the activity of these three neuronal populations with whole brain functional connectivity. Then we will target and stimulate the BF cholinergic neurons using optogenetics to understand how it influences network interactions and to identify the optimal conditions of stimulation in an AD animal model that can induce network states as observed during spontaneous activity in healthy animals. To achieve these goals, we propose a methodological approach that is both innovative and multimodal because it combines cutting edge techniques such as fMRI tools, optogenetics and fiber-optic calcium recording. The results of this study will provide an increased and better understanding of BF neural circuitry, thus opening new future perspectives for the treatment of cognitive disorders.

Researcher(s)

• Promoter: Keliris Georgios A.
• Co-promoter: Van Der Linden Annemie
• Co-promoter: Verhoye Marleen
• Fellow: Sitjà Roqueta Laia

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

Light is an important environmental factor driving important functions in animal physiology through the vision forming circuit or by non-image forming (NIF) brain circuit. NIF in mammals starts with melanopsin (OPN4) containing intrinsically photoreceptive retinal ganglionic cells (ipRGCs), that innervate brain regions regulating sleep, circadian functions, cognition etc. NIF has high sensitivity to blue light wavelength, which activates the melanopsin. Also, light through photoperiodic differences in seasons induces neuroplasticity in song bird's song control system (SCS), which may be hormone independent. We hypothesize that NIF processes underlie this neuroplasticity based on its influence in multiple brain regions not involved in visual system. To test this, we use European starling's, which show extensive SCS neuroplasticity in response to seasons. We want to first demonstrate that a NIF circuit exists in birds using in vivo MRI imaging along with molecular biology techniques with the contribution of eyes and brain in it. Then we propose to modulate the neuroplasticity by directly activating NIF through melanopsin by using blue light. Our results will for the first time link light and neuroplasticity. Melanopsin is also involved in disorders like Alzheimer's, seasonal affective disorder (SAD) and Light at night (LAN) related problems in cities. Thus, light, melanopsin and its influence on neuroplasticity may pave a way for further understanding of neurobiological disorders.

Researcher(s)

• Promoter: Van Der Linden Annemie
• Fellow: Majumdar Gaurav

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

Prior studies mainly focused on the effect of T on SCS plasticity. However, it has been shown that steroid-independent photostimulation can also induce SCS plasticity, but its mechanism remains unclear [9-11]. One of the proposed alternatives is the mediating effect of THs, as THs play an important role in the regulation of seasonal reproduction and are associated with neurogenesis. Surprisingly, the effect of THs on SCS plasticity has only been studied partially [24]. In addition, it is unknown whether THs mediate SCS plasticity in a direct or an indirect manner. To this end we designed a series of experiments divided into 3 work packages (WP).

Researcher(s)

• Promoter: Van Der Linden Annemie
• Fellow: Orije Jasmien

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

This project onderzoeken we of seizoenale neuroplastische veranderingen in sensorische netwerken van seizoenale zangvogels gepaard gaan met functionle veranderingen die leiden tot gedragswijzigingen die reproductie in de hand werken. Het onderzoek maakt gebruik van multisensory fMRI.

Researcher(s)

• Promoter: Van Der Linden Annemie
• Co-promoter: Keliris Georgios A.
• Fellow: Jonckers Elisabeth

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

The instrument acquired in this project is a matrix assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometer capable of mass spectrometry imaging (MSI). This technique is especially developed for the identification of biomolecules in a manner that retains cytological and histological patterning. This novel technical process, abbreviated to MALDI-MSI represents an interesting and extremely productive intersection between mass spectrometric and imaging platforms. Therefore, this grant is bridging 3 University of Antwerp CORE facilities (Center for Proteomics, Bio-Imaging lab and the Biomedical Microscopic Imaging Core). Using this MALDI-MSI platform, multiple research groups, brought together by a common interest in investigating molecular damage associated with aberrant aging mechanisms, will be able to identify a diverse range of small molecules (peptides and metabolites) as well as larger proteins directly on tissue slides, preserving the topological, histological and cytological data. This is not possible with routine proteomics and metabolomics technologies nor with advanced imaging techniques.

Researcher(s)

• Promoter: Maudsley Stuart
• Co-promoter: Baggerman Geert
• Co-promoter: Blust Ronny
• Co-promoter: Bogers John-Paul
• Co-promoter: Dewilde Sylvia
• Co-promoter: Husson Steven
• Co-promoter: Laukens Kris
• Co-promoter: Lemière Filip
• Co-promoter: Mertens Inge
• Co-promoter: Sobott Frank
• Co-promoter: Valkenborg Dirk
• Co-promoter: Van Der Linden Annemie
• Co-promoter: Van Ostade Xaveer

Research team(s)

Project type(s)

• Research Project

Abstract

This project investigates the role of hypothalamus-pituitary-thyroid hormons in the photoperiodicity induced neuroplasticity in seasonal songbirds and the interaction with hypothalamic-pituitary-gonadal axis hormones.

Researcher(s)

• Promoter: Van Der Linden Annemie

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

In this project, a new imaging methodology (functional Ultra Sound) is developed, tested and compared with resting state functional Magnetic Resonance Imaging to study the functional connectivity between different brain circuits.

Researcher(s)

• Promoter: Van Der Linden Annemie
• Co-promoter: Keliris Georgios A.
• Co-promoter: Verhoye Marleen

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

Alzheimer's disease (AD) is a devastating neurological disorder and the most common type of dementia in the elderly population. It is mainly characterised by the appearance of small protein aggregates inside the brain, commonly known as amyloid beta (Aß) plaques. These Aß plaques form when the soluble Aß peptides oligomerize, a process which was found to occur more frequently when high concentrations of soluble Aß are present. Very recently, a system responsible for the clearance of normal metabolic waste products and thus also the soluble Aß protein was identified inside the brain. This system, termed the glymphatic system (GS), was shown much more active during sleep compared to awake. As such, during sleep the GS clears waste products from the brain which formed during normal daytime activities. In this research project, we wish to investigate in more detail the relationship between sleep (and more specifically sleep disturbances), the GS, soluble Aß and the formation of Aß plaques. As sleep disturbances were seen to coincide with Aß plaque formation, we wish to elucidate which one of the 2 occurs first and, how this influences the GS. This is of particular interest to the general population as modern lifestyle considers sleep as a necessary evil with many people working during the night and even more sleeping too little too few, which could possibly result in sleep debt and thus an increased risk of developing AD.

Researcher(s)

• Promoter: Van Der Linden Annemie
• Fellow: Praet Jelle

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

I will study a white matter model that is not restricted to coherently-oriented structures, and parameterized by several white matter tract integrity metrics which are expected to be specific biomarkers of early pathologic changes. First, I will optimize the experimental design to enable accurate and precise parameter estimation. Second, a mouse model will be used to validate and understand what the model reflect at the microstructural tissue level. Third, I will evaluate whether the parameters are markers, capable in discriminating various pathological processes of Alzheimer disease.

Researcher(s)

• Promoter: Sijbers Jan
• Co-promoter: Van Der Linden Annemie
• Fellow: Veraart Jelle

Research team(s)

• Vision lab

Project type(s)

• Research Project

Abstract

In the framework of this project, a method will be established for automated and standardized microscopic evaluation of large numbers of biological samples. Protocols will be tailored for histological tissue preparations and for cell cultures. To this end, a combination of optics, robotics and bio-image informatics will be used.

Researcher(s)

• Promoter: De Vos Winnok
• Co-promoter: Bogers John-Paul
• Co-promoter: Kumar-Singh Samir
• Co-promoter: Maudsley Stuart
• Co-promoter: Timmerman Vincent
• Co-promoter: Van Der Linden Annemie

Research team(s)

• Laboratory of cell biology and histology

Project type(s)

• Research Project

Abstract

EGAMI stands for Expert Group Antwerp Molecular Imaging. Moreover, EGAMI is the mirror word of 'image'. EGAMI clusters the internationally recognized expertise in the profession of fundamental and biomedical imaging at the University of Antwerp: the Bio-Imaging Lab, the Molecular Imaging Center Antwerp (MICA), Radiology, the Laboratory for Cell Biology and Histology, and the Vision Lab (for post-processing of medical images). EGAMI's mission is providing an integrated research platform that comprises all aspects of multimodality translational medical imaging. Multimodality refers to the integration of information from the various imaging techniques. Within EGAMI, there is pre-clinical and clinical expertise and infrastructure for magnetic resonance imaging (MRI), computed tomography (CT), positron emission tomography (PET), and single-photon emission computed tomography (SPECT). EGAMI executes projects ranging from applied biomedical (imaging) and fundamental research to imaging methodologies. Die applied biomedical research focusses on the research fields neuro(bio)logy (i.e. development and validation of biomarkers (as well as therapy evaluation) for diseases like Alzheimer's, schizophrenia, multiple sclerosis etc.) and oncology (i.e. biomarkers for improved patient stratification and therapy monitoring). Since the pre-clinical biomedical research within EGAMI makes use of miniaturized versions of imaging equipment for humans (scanners) is it inherently translational, in other words initial findings acquired in animal experiments can be translated into clinical applications for improved diagnosis and treatment of patients ('from bench to bedside'). Beside the application of imaging in the biomedical research, EGAMI also conducts projects that aim to achieve an improvement and optimization of the imaging methodology. The expertise of the MICA (e.g. the development of new radiotracers) and of the Vision Lab (e.g. the development of image reconstruction, segmentation, and analysis algorithms) offers here the strategic platform to assemble intellectual property rights.

Researcher(s)

• Promoter: Van Der Linden Annemie
• Co-promoter: Parizel Paul
• Co-promoter: Sijbers Jan
• Co-promoter: Staelens Steven
• Co-promoter: Timmermans Jean-Pierre
• Fellow: Guns Pieter-Jan
• Fellow: Lanens Dirk
• Fellow: Van Putte Wouter

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

The overall goal of project is to contribute new information that will greatly increase our understanding about underlying mechanisms of hypothalamus-driven normal, accelerated and pathological aging of the brain, with the focus on structural alterations and subsequent alterations of specific functional networks such as the default mode network (DMN) and its anticorrelated networks. Our results will provide fundamental knowledge for age dependent intrinsic network structural changes as a reference for pathological aging studies where altered DMN activity needs to be reliably differentiated from that observed in healthy aging.

Researcher(s)

• Promoter: Van Der Linden Annemie
• Co-promoter: Ponsaerts Peter

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

The goal of this project is to investigate in more detail the link between sleep disturbances, amyloid pathology, the glymphatic system and aging. For this we will make use of state of the art MRI, EEG, immunohistochemistry, MALDI, PCR and ELISA. The combination of these in vivo and ex vivo techniques will offer us the possibility to elucidate this link, and to determine whether the glymphatic system might be an attractive target for future AD therapy development.

Researcher(s)

• Promoter: Van Der Linden Annemie

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

This project represents a formal research agreement between UA and on the other hand the client. UA provides the client research results mentioned in the title of the project under the conditions as stipulated in this contract.

Researcher(s)

• Promoter: Ponsaerts Peter
• Co-promoter: Van Der Linden Annemie

Research team(s)

Project type(s)

• Research Project

Abstract

Alzheimer's disease (AD) is a progressive brain disease that causes cognitive alterations, memory loss and behavioral changes. Neuropathological hallmarks of AD include brain atrophy, and the presence of two different types of protein aggregations: tangles composed of hyperphosphorylated Tau and plaques consisting of amyloid. The neurodegenerative process in AD is initially characterized by synaptic damage accompanied by neuronal loss. Moreover, synaptic loss is one of the strongest correlates to the cognitive impairment in patients with AD. The mechanisms underlying synaptic and cognitive deficits in AD remain poorly understood. To gain further insights into these mechanisms, we will examine how the brain changes in mutant Tau mice and how Tau is involved in cognitive and behavioral processes and related brain areas. Recent studies have suggested that targeting Tau is therapeutically promising. This will be done by compounds that dephosphorylate Tau. The different compounds will be evaluated by correlating behavioral with electrophysiological outputs, and state of the art in situ visualization techniques.

Researcher(s)

• Promoter: Van Der Linden Annemie

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

Aging has profound effects on many cellular processes that predispose to neurodegeneration, impairment in cognitive function, as well as changes in brain functional connectivity networks (e.g. default mode network) and synaptic alterations. However, the key mechanisms orchestrating brain aging remain largely unknown. More and more findings in rodents and humans have established that inflammatory processes in the hypothalamus can contribute to neurodegeneration upon aging via reproductive (HPG) axis. However, the exact mechanisms by which (i) Inflammatory signalling in the hypothalamus contributes to the occurrence of age-related functional connectivity and synaptic alterations, and (ii) hypothalamic HPG signalling modulates age-related neurodegeneration and cognitive changes are not well understood and need further investigation. The main goals of this project are to investigate: (i) how deregulation of the HPG axis impacts brain networks that display aging decline, (ii) how hypothalamic inflammation is steering deregulation of HPG axis in healthy aging, accelerated aging and pathological aging, and (iii) how hypothalamic inflammatory responses become activated upon healthy, accelerated and pathological aging, with specific focus on cellular, connectional architecture of functional networks. This project will contribute new information that will greatly increase our understanding about underlying mechanisms of hypothalamus-driven systematic aging of the brain.

Researcher(s)

• Promoter: Van Der Linden Annemie
• Co-promoter: Ponsaerts Peter
• Co-promoter: Vanden Berghe Wim
• Fellow: Kara Firat

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

The general objective is to obtain insight in the contribution and working mechanism of two vital processes in the brain: 1) neuroprotection and 2) 17β-estradiol (E2) induced neuroplasticity. As we aim to focus on different expression patterns of Ngb both in vitro and in vivo, the first objective is to improve valid Ngb overexpressing and knock-out (KO) systems.

Researcher(s)

• Promoter: Dewilde Sylvia
• Co-promoter: Ponsaerts Peter
• Co-promoter: Van Dam Debby
• Co-promoter: Van Der Linden Annemie
• Fellow: Luyckx Evi

Research team(s)

Project type(s)

• Research Project

Abstract

Prior studies mainly focused on the effect of T on SCS plasticity. However, it has been shown that steroid-independent photostimulation can also induce SCS plasticity, but its mechanism remains unclear [9-11]. One of the proposed alternatives is the mediating effect of THs, as THs play an important role in the regulation of seasonal reproduction and are associated with neurogenesis. Surprisingly, the effect of THs on SCS plasticity has only been studied partially [24]. In addition, it is unknown whether THs mediate SCS plasticity in a direct or an indirect manner. To this end we designed a series of experiments divided into 3 work packages (WP).

Researcher(s)

• Promoter: Van Der Linden Annemie
• Fellow: Orije Jasmien

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

Zebra finches are widely used models to study vocal learning. Like human babies learn how to speak, zebra finch juveniles learn how to sing during a specific sensitive period. The aim of this project is to investigate the neural mechanisms behind this sensitive period by analyzing song production and perception using song recordings and in vivo functional MRI during auditory stimulation with various songs including songs with different social context. The same group of birds is studied at several time points during and after song learning to monitor changes occurring in the brain. Sensory-motor learning is investigated in males, that learn how to sing from an adult tutor, and sensory learning in females, that do not sing but to whom song is crucial for mate choice. In addition, the effect of social environment during the sensitive period for vocal learning will be investigated. Ultimately, this study can reveal the time window and spatial coordinates of crucial events in the brain during song development and thereby initiates the investigation of the underlying neural and molecular mechanisms of song development as a model for human vocal development.

Researcher(s)

• Promoter: Van Der Linden Annemie
• Fellow: Van Ruijssevelt Lisbeth

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

Alzheimer's disease (AD) is the most common form of dementia, with a high prevalence in the elderly population. Amyloid pathology and inflammatory cascades, which show toxic effects at the synapses, have been implied as possible driving forces behind AD. Gaining a deeper insight in these early events is crucial for a better understanding of the mechanisms that drive AD progression. In this project we focus on the interaction between synaptic deficits, amyloidosis and inflammation using resting-state functional Magnetic Resonance Imaging in a mouse model of amyloidosis..

Researcher(s)

• Promoter: Van Der Linden Annemie
• Co-promoter: Verhoye Marleen
• Fellow: Shah Disha

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

This project represents a formal research agreement between UA and on the other hand SAO. UA provides SAO research results mentioned in the title of the project under the conditions as stipulated in this contract.

Researcher(s)

• Promoter: Van Der Linden Annemie

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

BRAINPATH aims to build upon current developments in molecular imaging by creating an academic-industrial training and mobility network for the next revolution of imaging technology. Molecular in vivo imaging is a fertile area which combines expertise, state-of-art equipment and many disciplines and inter-sector work environments. Our goal is to better understand brain diseases and develop new preclinical imaging strategies. We believe optical imaging in particular represents a technology that has the potential to exploit further our knowledge in this area.

Researcher(s)

• Promoter: Van Der Linden Annemie

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

In this project we are interested what hormonal interactions are needed (fast neuronal and/or slow gonadal) to create the permissive circumstances for neuroplastic changes seen in seasonal breeders. We want to examine if starlings have a more 'juvenile' pattern of connectivity when they are capable of learning new songs and when they can discriminate auditory vocal signals better. We will do this by using real-time, non-invasive, in vivo imaging tools that allow the follow-up of anatomical and functional changes of the songbird brain. The study of the reactivation of juvenile-like plasticity may potentially lead to a recovery of function in a variety of neurodevelopmental disorders.

Researcher(s)

• Promoter: Van Der Linden Annemie
• Fellow: De Groof Geert

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

We will follow the seasonal plasticity in SCS nuclei of male starlings with appropriate MRI tools and investigate the expression of receptors and enzymes that are essential in T and TH function in the SCS. Combining data from control birds and birds where T and TH availability has been manipulated will allow us to identify their individual impact as well as unravel their interactions.

Researcher(s)

• Promoter: Van Der Linden Annemie

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

In this project we want to tackle the question of how brain plasticity is regulated at the molecular level in two songbird models with different song learning and neuroplasticity characteristics, with the help of recently developed tools in brain imaging and epigenetics. Our hypothesis is that hormone and environment (light) induced effects contribute to brain plasticity at least in part through epigenetic programming.

Researcher(s)

• Promoter: Van Der Linden Annemie

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

This project represents a formal research agreement between UA and on the other hand Janssen Pharmaceutica. UA provides Janssen Pharmaceutica research results mentioned in the title of the project under the conditions as stipulated in this contract.

Researcher(s)

• Promoter: Van Der Linden Annemie
• Co-promoter: Sijbers Jan
• Co-promoter: Verhoye Marleen

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

Zebra finches are widely used models to study vocal learning. Like human babies learn how to speak, zebra finch juveniles learn how to sing during a specific sensitive period. The aim of this project is to investigate the neural mechanisms behind this sensitive period by analyzing song production and perception using song recordings and in vivo functional MRI during auditory stimulation with various songs including songs with different social context. The same group of birds is studied at several time points during and after song learning to monitor changes occurring in the brain. Sensory-motor learning is investigated in males, that learn how to sing from an adult tutor, and sensory learning in females, that do not sing but to whom song is crucial for mate choice. In addition, the effect of social environment during the sensitive period for vocal learning will be investigated. Ultimately, this study can reveal the time window and spatial coordinates of crucial events in the brain during song development and thereby initiates the investigation of the underlying neural and molecular mechanisms of song development as a model for human vocal development.

Researcher(s)

• Promoter: Van Der Linden Annemie
• Fellow: Van Ruijssevelt Lisbeth

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

This project represents a formal research agreement between UA and on the other hand the Flemish Public Service. UA provides the Flemish Public Service research results mentioned in the title of the project under the conditions as stipulated in this contract.

Researcher(s)

• Promoter: Stroobants Sigrid
• Co-promoter: Peeters Marc
• Co-promoter: Sijbers Jan
• Co-promoter: Staelens Steven
• Co-promoter: Van Der Linden Annemie

Research team(s)

Project type(s)

• Research Project

Abstract

The goal of this project (INMiND) is to carry out collaborative research on molecular mechanisms that link neuroinflammation with neurodegeneration in order to identify novel biological targets for activated microglia, which may serve for both diagnostic and therapeutic purposes, and to translate this knowledge into the clinic.

Researcher(s)

• Promoter: Van Der Linden Annemie

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

Advanced methods in Magnetic Resonance Imaging, such as resting state functional MRI (rfMRI) and Diffusion Tensor Imaging (DTI) will be used to study the effect of microgravity on the adaptive processes in the brain in astronauts. Preand post-flight data will be collected to elucidate changes in structural and functional brain wiring due to microgravity.

Researcher(s)

• Promoter: Wuyts Floris
• Co-promoter: Parizel Paul
• Co-promoter: Sijbers Jan
• Co-promoter: Van de Heyning Paul
• Co-promoter: Van Der Linden Annemie

Research team(s)

• Lab for Equilibrium Investigations and Aerospace (LEIA)

Project type(s)

• Research Project

Abstract

MULTIMAR or Multidisciplinary Magnetic Resonance comprises a network of 10 Flemish and 9 'international' research groups active in multidisciplinary and complementary research with a focus on methodologies that emanate from the phenomenon of magnetic resonance. These groups form a network organized around electron magnetic resonance (EMR, Prof. Van Doorslaer UA coordinator), nuclear magnetic resonance (NMR, Dr. N. Van Nuland, coordinator), and (pre-clinical) biomedical magnetic resonance (Prof. U. Himmelreich, coordinator). Further strengthening of the collaborations in magnetic resonance research in Flanders is supported by various regional large scale facilities for spectroscopy and imaging. The 'international' partners possess the expertise, equipment and research interests, which are complementary to at least two Flemish partners so that scientific interaction and exchange is instigated beyond the disciplinary borders.

Researcher(s)

• Promoter: Van Der Linden Annemie

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

This project represents a research agreement between the UA and on the onther hand IWT. UA provides IWT research results mentioned in the title of the project under the conditions as stipulated in this contract.

Researcher(s)

• Promoter: Van Der Linden Annemie
• Fellow: Guglielmetti Caroline

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

The current project will follow the development of neuroinflammation together with functional brain integrity and behavioural outcome in a rodent model of maternal immune activation in vivo utilising state-of-the-art multimodal imaging biomarkers.This project will generate highly novel information about the contribution of neuroinflammation to the development of schizophrenia and its consequences for the functional integrity of the brain, and eventually provide a rationale for the implementation of novel disease-modifying strategies.

Researcher(s)

• Promoter: Dedeurwaerdere Stefanie
• Co-promoter: Staelens Steven
• Co-promoter: Timmermans Jean-Pierre
• Co-promoter: Van Der Linden Annemie

Research team(s)

• Translational Neurosciences (TNW)

Project type(s)

• Research Project

Abstract

The SuperMRI project aims at - speeding up the acquisition process by developing new imaging sequences and sparse sampling strategies - reducing the computation time of iterative reconstruction algorithms by developing fast and generic forward and backward projectors through parallelization and distribution of the algorithms, in combination with suitable hardware architecture (GPU or FPGA). - Significantly improving the image quality by developing novel reconstruction algorithms for MRI, related to compressive sensing and discrete tomography. - developing fast tomographic image processing algorithms that exploit the available k-space data, with focus on segmentation and motion compensation.

Researcher(s)

• Promoter: Van Der Linden Annemie

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

This project represents a research agreement between the UA and on the onther hand IWT. UA provides IWT research results mentioned in the title of the project under the conditions as stipulated in this contract.

Researcher(s)

• Promoter: Van Der Linden Annemie

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

In this PhD project we aim to completely characterize the migration behaviour, fate and physiology of different stem cell types following administration in the 'experimental autoimmune encephalomyelitis' (EAE) mouse model for Multiple Sclerosis. A combination of in vivo (BLI and MRI) and post-mortem (histology) imaging modalities will be used to reveal different cell characteristics and compare these characteristics between the different cell types included in this study. These results will enable us to effectively modify stem cell populations in order to enhance possible therapeutic effects (e.g. enhanced migration towards target sites and increased survival of grafted stem cells).

Researcher(s)

• Promoter: Van Der Linden Annemie
• Co-promoter: Ponsaerts Peter
• Fellow: De Vocht Nathalie

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

The aim of this project is to further develop uPA probes, of which we already showed the efficacy in in vitro studies, to be used in cellular and in vivo. The IP of these innovative probes have recently been submitted to the UA interface for patenting. The first step in the valorisation of the probes is to obtain proof of concept in in vivo disease models. In the subsequent phase these results will permit us to obtain further funding from larger public (Fournier-Majoie, IWT) or private (VC) institutions. Our goal is to proceed with spinning-out this te chnology into a company preferentially within 3 years.

Researcher(s)

• Promoter: Augustyns Koen
• Co-promoter: Bogers John-Paul
• Co-promoter: De Visscher Geofrey
• Co-promoter: Joossens Jurgen
• Co-promoter: Lardon Filip
• Co-promoter: Peeters Marc
• Co-promoter: Stroobants Sigrid
• Co-promoter: Van Der Linden Annemie

Research team(s)

• Medicinal Chemistry (UAMC)

Project type(s)

• Research Project

Abstract

This proposed multidisciplinary research consortium, consisting of 6 different laboratories from the University of Antwerp, aims to understand the cellular and/or functional interactions of NSC implants in healthy and injured neural tissue (cuprizone-mediated demyelinisation mouse model). With this research project, which focuses on the in vivo molecular and physiological control of NSC, we aim to contribute to the in vivo study and modulation of NSC migration, survival, differentiation and functional integration.

Researcher(s)

• Promoter: Berneman Zwi
• Co-promoter: Giugliano Michele
• Co-promoter: Jorens Philippe
• Co-promoter: Pauwels Patrick
• Co-promoter: Ponsaerts Peter
• Co-promoter: Van Der Linden Annemie
• Co-promoter: Ysebaert Dirk

Research team(s)

Project type(s)

• Research Project

Abstract

In some species of songbirds, the brain displays a pronounced seasonal rewiring which is associated with seasonal behavioural changes in vocal communication (singing only in spring). Both humans and songbirds learn their vocal communication and depend on speech- or song input during critical/sensitive periods in life in order to do so. For some bird species, like the zebra finch, this sensitive period is limited to a certain period after hatching (age-limited learner), in others, like the canary, this sensitive period is re-opened seasonally (open-ended learner). Longitudinal studies which obtain information both on structure and activity of the brain in living birds (using neuro-imaging) and at the same time their behaviour (singing/learning) create the unique opportunity to detect relevant moments of behaviourally related neuronal circuit changes enabling not only cause-and-effect relationship- but also target definition for subsequent gene expression studies. This information will contribute in unravelling the molecular mechanisms of neural circuitry rewiring and speech learning processes both with relevance for regenerative medicine in general and for children who, because of a history of deafness, missed out on the input they needed to learn their language properly in particular.

Researcher(s)

• Promoter: Van Der Linden Annemie
• Co-promoter: De Visscher Geofrey

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

In this project, we aim to further elucidate the mechanisms leading to immune-mediated rejection of allografts in the CNS. For this, we will non-invasively (by in vivo bioluminescence imaging) identify the exact timing and degree of microglia immune-reactivity in relation to immune-mediated rejection of different allogeneic adult-, embryo- and placenta-derived cell populations.

Researcher(s)

• Promoter: Berneman Zwi
• Co-promoter: Pauwels Patrick
• Co-promoter: Ponsaerts Peter
• Co-promoter: Van Der Linden Annemie
• Co-promoter: Ysebaert Dirk

Research team(s)

Project type(s)

• Research Project

Abstract

Songbirds exhibit seasonal plasticity in a broad variety of behavioral and morphological traits associated with reproduction. Changes in song production are well described while changes in song perception are not. In the present project, we will study the seasonal functional variation in auditory processing of the European starling (Sturnus vulgaris) using functional magnetic resonance imaging. We will test if the neural substrates of song perception will differ according to the function and the social value of the songs and if seasonal changes of these neural substrates will be observed only for the songs whose function and social value change seasonally. Because we suspect steroids to be involved in the seasonal changes we measure, the neural substrates of song perception will also be investigated after administering steroids. We expect at least some of the seasonal changes to be reproduced by steroid hormones, providing a causal link between steroids and perceptual sensory plasticity. An anatomical seasonal plasticity in the visual system's optic chiasm has also been identified in starlings. The exact ultrastructural explanation/mechanism behind this remarkable change however remains unknown. In a second part of this project we will study the exact mechanisms responsible for seasonal anatomical variation in the optic chiasm.

Researcher(s)

• Promoter: Van Der Linden Annemie
• Fellow: De Groof Geert

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

This project has a double purpose. The first objective is implementation and optimization of 'resting state fRMI' in control rats as a proof of principle. Most of the research is done in humans and only a few studies on animals are reported. It will be a challenge to develop a scanning and analysis protocol with optimal parameters for scanning, anesthesia and data-processing. Subsequently the implementation and optimization will be extended to songbirds. The second objective is to understand the accordance of low frequent fluctuations measured during resting state in different brain regions and the anatomical and functional connectivity of these regions (as observed with DTI en MEMRI).

Researcher(s)

• Promoter: Van Der Linden Annemie
• Fellow: Jonckers Elisabeth

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

Magnetic Resonance imaging plays a crucial role in stem cell research in order to investigate whether administered stem cells are able to migrate to the target organ, locally survive, differentiate and contribute to regenerated tissue. However, knowledge regarding the interaction of MRI contrast agents with (sub)cellular structures is lacking. In this project, we will use advanced TEM techniques to investigate different MRI contrast agents and loading techniques for neural stem cells.

Researcher(s)

• Promoter: Bals Sara
• Co-promoter: Ponsaerts Peter
• Co-promoter: Van Der Linden Annemie

Research team(s)

• Electron microscopy for materials research (EMAT)

Project type(s)

• Research Project

Abstract

This project represents a research contract awarded by the University of Antwerp. The supervisor provides the Antwerp University research mentioned in the title of the project under the conditions stipulated by the university.

Researcher(s)

• Promoter: Van Der Linden Annemie
• Co-promoter: Adriaensen Dirk
• Co-promoter: Timmermans Jean-Pierre
• Fellow: De Visscher Geofrey
• Fellow: Guns Pieter-Jan

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

The overall objective of the project is to develop an in vivo BLI/MRI model for experimental studies on human African trypanosomiasis that allows high resolution localisation of parasites and associated inflammatory reactions during tissue invasion.

Researcher(s)

• Promoter: Van Der Linden Annemie

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

This project represents a formal research agreement between UA and on the other hand IBBT. UA provides IBBT research results mentioned in the title of the project under the conditions as stipulated in this contract.

Researcher(s)

• Promoter: Van Der Linden Annemie

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

This project represents a research agreement between the UA and on the onther hand IWT. UA provides IWT research results mentioned in the title of the project under the conditions as stipulated in this contract.

Researcher(s)

• Promoter: Van Der Linden Annemie
• Fellow: Blockx Ines

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

This is a fundamental research project financed by the Research Foundation - Flanders (FWO). The project was subsidized after selection by the FWO-expert panel.

Researcher(s)

• Promoter: Van Der Linden Annemie
• Fellow: van der Kant Anne

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

The aim of this project is to study how different amplitude modulation rates are processed in the ascending auditory cortex of macaques and songbirds and to determine if this processing is lateralised. To answer these questions, functional Magnetic Resonance Imaging (fMRI) will be used. The work will shed new light on fundamental mechanisms of auditory temporal analysis of general relevance to the analysis of ethological sounds in different species.

Researcher(s)

• Promoter: Van Der Linden Annemie
• Fellow: Poirier Colline

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

This is a fundamental research project financed by the Research Foundation - Flanders (FWO). The project was subsidized after selection by the FWO-expert panel.

Researcher(s)

• Promoter: Van Der Linden Annemie
• Co-promoter: Ponsaerts Peter
• Fellow: De Vocht Nathalie

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Van Der Linden Annemie
• Fellow: Verschueren Jacob

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

This project represents a research contract awarded by the University of Antwerp. The supervisor provides the Antwerp University research mentioned in the title of the project under the conditions stipulated by the university.

Researcher(s)

• Promoter: Van Der Linden Annemie

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

The main goal of this project is to shed light on the causal relationship between steroid hormones (testosterone), brain plasticity (of the song control system and other relevant regions), motor behavior (song) and (sexual) motivation by making use of the newest possibilities offered by MR-imaging. All experiments will be conducted with the starling (Sturnus vulgaris) as model species. Three hypotheses will be tested: 1. Testosterone acts directly on the song control system and is the cause of its growth and higher song production, 2. Testosterone does not act directly on the song control system but on other regions in the brain who are involved in sexually motivated behavior, 3. The higher (motor) activity (song) induces brain plasticity.

Researcher(s)

• Promoter: Van Der Linden Annemie
• Fellow: De Groof Geert

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

In this study, we aim to investigate whether genetic modification of exogenous stem cells with chemokine receptors enhances their migration towards lesions in brain tissue. Magnetic resonance imaging (MRI) and bioluminescence imaging (BLI) technology will be used and validated to visualize migration and survival of grafted stem cells in vivo.

Researcher(s)

• Promoter: Van Der Linden Annemie
• Co-promoter: Ponsaerts Peter
• Fellow: De Vocht Nathalie

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

This project represents a research contract awarded by the University of Antwerp. The supervisor provides the Antwerp University research mentioned in the title of the project under the conditions stipulated by the university.

Researcher(s)

• Promoter: Van Der Linden Annemie

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

With the aging of the population, degenerative and ischemic disorders are becoming an increasing economic and social burden. The characterization over the last decade of tissue specific stem cells other than hematopoietic stem cells (HSCs) including neural stem cells, mesenchymal stem cells and others, as well as pluripotent stem cells such as embryonic stem cells (ESCs) or multipotent adult progenitor cells (MAPCs) offers the possibility that stem cells may be used to treat disorders caused by degeneration or ischemia. The major advantage of HSC therapy is that the fate of the cells and their progeny can be readily followed by simple analysis of circulating blood or bone marrow biopsies. By contrast, the fate of stem cells resident in or grafted in solid organs can not be readily followed. Hence one of the major impediments to determine if stem cells might be exploited to treat disorders of solid organs is the inability to follow the fate (such as migration, survival and lineage differentiation) of stem cells, whether endogenous to the affected organ or grafted in the organ, in vivo using non-invasive means. Therefore, we have assembled a group of investigators from the K.U.LEUVEN, UNIVERSITEIT ANTWERPEN and UNIVERSITEIT GENT, who are recognized worldwide for their expertise in respectively stem cell research, non-invasive imaging technology and micro-& nanomaterials for biomedical and pharmaceutical purposes .. The consortium will develop methods to manipulate endogenous stem cells as well as cultured multipotent stem cell populations that can be grafted to enable non-invasive imaging of migration and survival of the cells in vivo, and to also enhance migration and survival. In a second platform multimodality imaging will be developed to allow in a non-invasive manner to follow the fate of stem cells in vivo. Some of these imaging modalities, here focused around stem cells, should be readily translatable to the clinic both to follow stem cell fate, but also outside of the area of stem cell research as we believe that some of the technical optimization of CT-scan, PET-scan and MRI based non-invasive imaging should have much broader applications. Moreover, development of genetic and direct labeling methods of stem cells to allow following their fate as well as modify their fate should prove very useful for studies aimed at testing the effect of drugs on stem cell or more differentiated cell behavior in vivo. Thus: although stroke will be used as the model disease, and MSCs, MAPCs and endogenous NSCs are the cells to be modified, this technology will constitute a generic but innovative set of methods that can then be used in other disease models, employing other stem cell populations, and outside the field of stem cell based and derived therapies.

Researcher(s)

• Promoter: Van Der Linden Annemie

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

In healthy adult mice the major location with the production of neural precursor cells is 'the subventricular zone (SVZ' located near the later ventricles of the brain. From this region, the neural precursor cells migrate towards the olfactory bulb where they differentiate into new interneurons. This migration occurs in healthy animals along a specific pathway, the Rostral Migration Stream (RMS). In vivo examination of this normal migration is the first step towards possible manipulation of endogenous neural precursor cells for therapeutic purposes. For the in situ labeling of the precursor cells with a superparamagnetic contrast agent, we will evaluate and compare two new labeling techniques: 1)Direct injection of superparamagnetic iron oxide particles (SPIO's) into the lateral ventricle so that the SVZ cells after uptake of the SPIO's will be visible with in vivo MRI. 2)Viral introduction of a MRI reportergen into the SVZ cells by which the cells will produce a superparamagnetic contrast agent and can be visualized by MRI. The successful design of a cellular imaging study with in vivo MRI depends on series of methodological evaluations and optimizations. The evaluation of both labeling techniques will be based on: 1) toxicity and effect on the cell proliferation and migration of the contrast agent and the reportergen, 2) the identification of the cell types by which the contrast agent is taken up/produced, 3) the visualization of the neuronal migration with in vivo MRI and 4) the visualization of incorporated new neurons in the olfactory bulb. The validation of the MRI studies will be done by immunohistochemistry.

Researcher(s)

• Promoter: Van Der Linden Annemie
• Fellow: Vreys Ruth

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Van Der Linden Annemie
• Fellow: Blockx Ines

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

This project aims at visualising and quantifying endogenous neuronal stem cell recruitment in the brain of living songbirds using Bioluminescent Imaging (BLI) with the reporter gene luciferase and Magnetic Resonance Imaging (MRI) using either the reporter gene Ferritine or MRI contrast agents that are internalised by the neuronal progenitor cells in the subventricular zone. The developed tools will then be used to study the underlying mechanisms of seasonal changes in neuronal recruitment.

Researcher(s)

• Promoter: Van Der Linden Annemie

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

To gain insight in the effect of neurodegeneration on the functioning of neuronal networks in HD and SCA17 by the application of complementary in vivo imaging techniques that (1) specifically provide information at the molecular level ¿ receptors and metabolism ¿ using ¿PET imaging; that (2) are able to measure axonal transport rates and neuronal connections using MEMRI; and that (3) allow to study the downstream effects within a neuronal network after systemic administration of a a- /antagonist using phMRI.

Researcher(s)

• Promoter: Van Der Linden Annemie
• Fellow: Van Camp Nadja

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

This project represents a formal research agreement between UA and on the other hand the Federal Public Service. UA provides the Federal Public Service research results mentioned in the title of the project under the conditions as stipulated in this contract.

Researcher(s)

• Promoter: Van Der Linden Annemie

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

This is a fundamental research project financed by the Research Foundation - Flanders (FWO). The project was subsidized after selection by the FWO-expert panel.

Researcher(s)

• Promoter: Van Der Linden Annemie

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Eens Marcel
• Co-promoter: Van Der Linden Annemie

Research team(s)

• Behavioural Ecology & Ecophysiology

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Dommisse Roger
• Co-promoter: Van Der Linden Annemie

Research team(s)

Project type(s)

• Research Project

Abstract

The RATstream' consortium will concentrate on the comprehensive phenotypical characterization of rat models of neurodegenerative diseases such as Huntington's disease (HD), Parkinson's disease (PD) and spinocerebellar ataxia type 17 (SCA17). Ultimately, the project will deliver a procedure for low cost automated drug screening along with a set of data describing the phenotype for each of the models. To achieve this goal, automated home cage systems for behavioural and physiological phenotyping will be developed by two SMEs and validated independently by two academic partners and individual data will be incorporated into an integrated database developed by a third SME. In a joint effort the groups will develop a comprehensive set of behavioural and physiological phenotyping procedures including PET and DTI technologies to systematically detect neuropsychiatric correlates of neuronal dysfunction and disease progression in rat models of HD, PD, and SCA17. The resulting set of biomarkers will lead to a valid set of minimised experiments and markers best suited to provide read-out parameters in pre-clinical studies applying novel substances delaying or preventing neurodegeneration.

Researcher(s)

• Promoter: Van Der Linden Annemie

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Van Der Linden Annemie

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

Stem cell transplantation after neurotrauma is a promising field of research in current biomedical research. However, to date, little is known about successful migration and survival of transplanted stem cell populations on site of trauma. This project aims to develop genetically modified adult and embryonic stem cell populations which can be assessed by MRI and BLI after transplantation in traumatised mouse brain.

Researcher(s)

• Promoter: Van Der Linden Annemie
• Co-promoter: Ponsaerts Peter

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Van Der Linden Annemie
• Fellow: Van Meir Vincent

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Van Der Linden Annemie
• Fellow: De Groof Geert

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

Songbirds (i.e. Passeriformes) share with humans the capacity of vocal imitation. Like human speech is, is birdsong a complex natural learned behavior that requires memorization of the song of an adult tutor. The acquisition and production of songs is controlled by a circuit of brain regions, the song control system (SCS). Also the auditory system, responsible for perception and processing of auditory information, is critical for song learning. Song learning inquires hearing, listening and memorizing songs, intrinsically suggesting an active role of the auditory system. Although the auditory pathways are far less explored than the vocal pathways, phenomena's like habituation and hierarchical organization were determined in the auditory system. More evidence for a functional connection between the two circuits is the indication that parts of the auditory system (and not of the SCS!) act as the neural substrate for song recognition memory (i.e. the so called tutor template). In clinical research, the neural substrate for speech and language is examined with in-vivo functional MRI in order to localize the auditory and speech induced activations in the human brain. The successful implementation of this method in songbirds (Van Meir et al., 2004) allows us to visualize the auditory brain activity in the auditory and/or vocal circuit in anaesthetized animals. This will allow us to detect for brain activity during exposure to different auditory stimulations (for example conspecific and birds' own song, manipulated song, multi tones,') within the same individual and even before and after song learning (tutoring birds). Thanks to the combination with experimental animal research, underlying cellular and molecular mechanisms of cognition can be investigated. The purpose of the project is to determine the neural substrate that is necessary for auditory recognition in songbirds. Is this region related to the conventional song circuit? When we analyze with fMRI the neuronal activation in zebra finches with exposure to learned song and other acoustic stimuli in relation to the degree of song learning, the neural substrate in songbirds that is involved with auditory recognition can be investigated.

Researcher(s)

• Promoter: Van Der Linden Annemie
• Fellow: Boumans Tiny

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

This project represents a research contract awarded by the University of Antwerp. The supervisor provides the Antwerp University research mentioned in the title of the project under the conditions stipulated by the university.

Researcher(s)

• Promoter: Van Der Linden Annemie

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

In healthy adult mice the major location with the production of neural precursor cells is 'the subventricular zone (SVZ' located near the later ventricles of the brain. From this region, the neural precursor cells migrate towards the olfactory bulb where they differentiate into new interneurons. This migration occurs in healthy animals along a specific pathway, the Rostral Migration Stream (RMS). In vivo examination of this normal migration is the first step towards possible manipulation of endogenous neural precursor cells for therapeutic purposes. For the in situ labeling of the precursor cells with a superparamagnetic contrast agent, we will evaluate and compare two new labeling techniques: 1) Direct injection of superparamagnetic iron oxide particles (SPIO's) into the lateral ventricle so that the SVZ cells after uptake of the SPIO's will be visible with in vivo MRI. 2) Viral introduction of a MRI reportergen into the SVZ cells by which the cells will produce a superparamagnetic contrast agent and can be visualized by MRI. The successful design of a cellular imaging study with in vivo MRI depends on series of methodological evaluations and optimizations. The evaluation of both labeling techniques will be based on: 1) toxicity and effect on the cell proliferation and migration of the contrast agent and the reportergen, 2) the identification of the cell types by which the contrast agent is taken up/produced, 3) the visualization of the neuronal migration with in vivo MRI and 4) the visualization of incorporated new neurons in the olfactory bulb. The validation of the MRI studies will be done by immunohistochemistry.

Researcher(s)

• Promoter: Van Der Linden Annemie
• Fellow: Vreys Ruth

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

Diffusion Tensor Magnetic Resonance Imaging (DT-MRI) is a recently developed technique who permits to study the architecture of white brainmatter (WM) in vivo and in an non-invasive way. DT-MRI is based on the Brownian movement of H2O-molecules in biological tissue and makes it possible to determine the anisotropic diffusion of these molecules . This anisotropic diffusion can be related to aligned microstructures, like WM brain fibres, which has a great value in biomedical applications. Since a large amount of data is needed for this technique, it is desirable to use fast imaging sequences. However, these kind of sequences introduce specific artefacts in the images which degrade the quality of the DT-measurements. For this reason, several strategies will be used to upgrade this quality. The present acquisition standard for fast DTI, Echo Planar Imaging (EPI), is prone to severe susceptibility artefacts which introduce geometric distortions in the images. These artefacts are more explicit when working at higher field strengths (here: 7 Tesla and 9.4 Tesla). By using an adapted EPI-sequence, it is possible to measure the local susceptibility artefacts and to correct for distortions. Another strategy that will be used is to combine DTI with Fast Spin Echo (FSE). This technique should be less sensitive to susceptibility artefacts. A recent approach, in which multiple receivers are used (Parallel Imaging) will be used to reduce artefacts in DT-MRI.

Researcher(s)

• Promoter: Van Der Linden Annemie
• Fellow: Pintjens Wouter

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

The main question that will be studied during the proposed experiments is the analysis of the role played by the catecholaminergic innervation of the auditory and song control areas in the control of auditory processing in songbirds. Special attention will be given first to norepinephrine (NE), the catecholamine that is most likely associated with the modulation of sound processing. Future studies should also consider the potential role of dopamine.

Researcher(s)

• Promoter: Van Der Linden Annemie

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Van Der Linden Annemie

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

Songbirds share with humans the capacity to produce learned vocalizations (song) and the neural substrate for song learning and production displays a remarkable seasonal plasticity. It is our aim to study these neuroplastic features, using in-vivo MRI and functional MRI of songbirds and experimentally manipulated songbirds, in order to unravel the pattern of neuroplasticity in relation to seasonal changes in song perception, learning and song production in songbirds.

Researcher(s)

• Promoter: Van Der Linden Annemie

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

Gelieve aan te vullen a.u.b.

Researcher(s)

• Promoter: Sijbers Jan
• Co-promoter: Van Der Linden Annemie
• Fellow: Leemans Alexander

Research team(s)

• Vision lab

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Van Der Linden Annemie

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Van Der Linden Annemie

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

Songbirds (i.e. Passeriformes) share with humans the capacity of vocal imitation. Like human speech is, is birdsong a complex natural learned behavior that requires memorization of the song of an adult tutor. The acquisition and production of songs is controlled by a circuit of brain regions, the song control system (SCS). Also the auditory system, responsible for perception and processing of auditory information, is critical for song learning. Song learning inquires hearing, listening and memorizing songs, intrinsically suggesting an active role of the auditory system. Although the auditory pathways are far less explored than the vocal pathways, phenomena's like habituation and hierarchical organization were determined in the auditory system. More evidence for a functional connection between the two circuits is the indication that parts of the auditory system (and not of the SCS!) act as the neural substrate for song recognition memory (i.e. the so called tutor template). In clinical research, the neural substrate for speech and language is examined with in-vivo functional MRI in order to localize the auditory and speech induced activations in the human brain. The successful implementation of this method in songbirds (Van Meir et al., 2004) allows us to visualize the auditory brain activity in the auditory and/or vocal circuit in anaesthetized animals. This will allow us to detect for brain activity during exposure to different auditory stimulations (for example conspecific and birds' own song, manipulated song, multi tones,') within the same individual and even before and after song learning (tutoring birds). Thanks to the combination with experimental animal research, underlying cellular and molecular mechanisms of cognition can be investigated. The purpose of the project is to determine the neural substrate that is necessary for auditory recognition in songbirds. Is this region related to the conventional song circuit? When we analyze with fMRI the neuronal activation in zebra finches with exposure to learned song and other acoustic stimuli in relation to the degree of song learning, the neural substrate in songbirds that is involved with auditory recognition can be investigated.

Researcher(s)

• Promoter: Van Der Linden Annemie
• Fellow: Boumans Tiny

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Van Der Linden Annemie

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Van Der Linden Annemie

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

The project involves the development of an alternative for the development of transgenic animal models, with focus on neurodegenerative diseases. The basis principle is the use of lenti viral vectors for systematic suppression of different genes by RNA inhibition. Non invasive bio-imaging methods such as in-vivo micro PET, micro SPECT, micro CT , micro MRI and bioluminescence will be developed and implemented on small animals Besides transduction of adult differentiated cells, also the potential of stem cell migration will be tackled in the developed models.

Researcher(s)

• Promoter: Van Der Linden Annemie

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

): The use of Echo Planar Imaging (EPI) as a rapid Magnetic Resonance Imaging (MRI) technique, b1Jth in human as animal brain research, is susceptible to geometric distortions. In the frame of this project, new developments -both on the level of MR image acquisition and MR image processing will be made to correct for these EPI-distorsions. The developped correction technique will be implemented and validated in functional MRI (fMRI) studies perfonned in rats and songbirds.

Researcher(s)

• Promoter: Verhoye Marleen
• Co-promoter: Van Der Linden Annemie

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Van Der Linden Annemie

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

We recently identified a novel mutation in an Alzheimer's disease (AD) gene that leads to deposition of brain amyloid ß (Aß) as unusual diffuse plaques, rather than the classical compact plaques. One of the most severe AD features present in these patients fits well with recent in vitro data suggesting that early Aß conformational states could be the real culprit. We intend to test this hypothesis on a correlative neuropathological, biochemical, and neuro MRI studie on mice overexpressing this mutant gene.

Researcher(s)

• Promoter: Kumar-Singh Samir
• Co-promoter: Van Der Linden Annemie

Research team(s)

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Van Der Linden Annemie
• Fellow: Tindemans Ilse

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Van Der Linden Annemie

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Van Der Linden Annemie

Research team(s)

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Van Der Linden Annemie
• Fellow: Van Camp Nadja

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Eens Marcel
• Co-promoter: Van Der Linden Annemie
• Fellow: Van Meir Vincent

Research team(s)

• Behavioural Ecology & Ecophysiology

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Van Der Linden Annemie
• Fellow: Vanhoutte Greetje

Research team(s)

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Van Der Linden Annemie

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Van Der Linden Annemie

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Van Der Linden Annemie
• Fellow: Steendam Gwendy

Research team(s)

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Van Der Linden Annemie
• Fellow: Van Der Linden Annemie

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Van Der Linden Annemie

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Van Der Linden Annemie

Research team(s)

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Van Der Linden Annemie
• Fellow: Verhoye Marleen

Research team(s)

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Van Der Linden Annemie

Research team(s)

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Van Der Linden Annemie

Research team(s)

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Van Der Linden Annemie
• Fellow: Tindemans Ilse

Research team(s)

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Van Der Linden Annemie

Research team(s)

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Van Der Linden Annemie
• Fellow: Van Camp Nadja

Research team(s)

Project type(s)

• Research Project

Abstract

Notwithstanding the simple and regular anatomical organisation of the cerebellum, its function and activity remains unclear. This project aims at better understanding the function and activity of the cerebellum applying computer simulations and experiments in both men and animals. To that end morphological and electrophysiological techniques will be used on rats and functional magnetic resonance imaging will be performed on rat and men.

Researcher(s)

• Promoter: Van Der Linden Annemie
• Co-promoter: Timmermans Jean-Pierre

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Van Der Linden Annemie

Research team(s)

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Van Der Linden Annemie
• Fellow: Eelen Jan

Research team(s)

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Van Der Linden Annemie
• Fellow: Tindemans Ilse

Research team(s)

Project type(s)

• Research Project

Abstract

A few vertebrates have access to biochemical and physiological strategies that allow their brain to be anoxia resistant. In the framework of this project we want to investigate the underlying mechanisms. To that end magnetic resonance imaging and spectroscopy will be performed, focussing the brain, on living common and crucian carp under circumstances of hypoxia and anoxia.

Researcher(s)

• Promoter: Van Der Linden Annemie
• Co-promoter: Blust Ronny

Research team(s)

Project type(s)

• Research Project

Abstract

Implementation and optimalization of high resolution fMRI and image analysis on a 7 Tesla machine, using different acquisition and processing techniques, with the aim of achieving a high spatial as well as temporal resolution.

Researcher(s)

• Promoter: Van Dyck Dirk
• Co-promoter: Van Der Linden Annemie
• Fellow: Peeters Ronald

Research team(s)

Project type(s)

• Research Project

Abstract

Within this project we will study the endocrinological origin of individual differences in reproductive behavior. Also the effects of testosterone on the development of secondary sexual characteristics, on physiological processes, the immune systeme and ultimately survival will be investigated. The following bird models will be used: starling, titmouse, water-hen and paroquet.

Researcher(s)

• Promoter: Van Der Linden Annemie
• Co-promoter: Verheyen Rudi

Research team(s)

Project type(s)

• Research Project

Abstract

The objective of this project is to develop hardware and software which allows the simultaneous acquisition of MRI and EEG in both an animal and a human research environment. This will result in an EEG upgrade of the MRI instrument.

Researcher(s)

• Promoter: Van Der Linden Annemie

Research team(s)

Project type(s)

• Research Project

Abstract

It is the major function of cutaneous blood flow to control the loss of heat from the body surface. In the rat, almost 20% of the total body heat-loss occurs by sympathetically mediated increases in blood flow through a system of arteriovenous anastomoses (AVAs) in the skin of the tail which are absent at the base and abundant at the tip. The mechanisms which are really involved in thermoregulation of the rat remained unsolved since none of the work cited, approaches both temperature and blood vessel size. Our aim was to monitor online the blood vessel temperature, as measured externally, and the arterial and venous vessel size and their mutual vascular volume interactions using in vivo MRA. Application: multi slice gradient echo sequence with TE/TR 6/20ms, FOV 20mm. The experiment protocol was repeated at 3 important regions of the tail: base, middle and tip during a gradual rise of rectal temperature from 36°C to 40°C. We measured also tail blood temperature applying temperature dependent resistors attached with adhesive tape and carefully positioned at the ventral artery (Ta) and lateral vein (Tv). In all cases, MRA data sets yielded a perfect representation of the tail's vascular anatomy (its 3 major axial artery-vein pairs, one ventral and two lateral). The diameter of the ventral artery and the lateral veins of the heat-loaded animal increased clearly. The size of the 3 smaller veins of the ventral artery-vein pair increased only beyond a rectal temperature of 39°C. Calculation of (Ta-Tv) in function of the rectal temperature during heating, learnt more about the thermogenetic vaso-activity effect of the tail blood vessels and the onset of vasodilation. At the tail base, a maximum difference was observed at rectal temperature of 38°C and a minimum at 39°C. At the middle and the tip of the tail, we observed a steady rise of (Ta-Tv). If we assume that vasodilatation is a synchronical process along the length of the tail, then the difference in (Ta-Tv) is due to the presence of AVAs.

Researcher(s)

• Promoter: Raman Erik
• Co-promoter: Van Der Linden Annemie

Research team(s)

Project type(s)

• Research Project

Abstract

This research proposal addresses the investigation of the anoxia resistance of the brain of crucian carp (Carassius carassius). In-vivo Nuclear Magnetic Resonance imaging and spectroscopy will be applied on living intact carps under circumstances of hypoxia and anoxia. This will provide insight in 1) the adaptations of the haemoglobin with reference to the oxygen affinity, 2) alterations in bloodperfusion in the brain and 3) energy- ion- and water household of the brain.

Researcher(s)

• Promoter: Van Der Linden Annemie

Research team(s)

Project type(s)

• Research Project

Abstract

Some vertebrates, such as the crucian carp, the American brown frog and some fresh water turtles possess biochemical and physiological strategies which allow them to adapt circumstances of anoxia, a circumstance which is fatal for the brain of all other vertebrates. Using in-vivo MRI and MRS we will investigate the reactions of the brain tissue, related to cell homeostasis and energy content, upon axonic and hypoxic conditions, as a function of time.

Researcher(s)

• Promoter: Van Der Linden Annemie
• Co-promoter: Blust Ronny

Research team(s)

Project type(s)

• Research Project

Abstract

Freshwater animal possess regulatory mechanisms to maintain there ion and water homeostasis without large alterations in their energy expenditure. Exposure to environmental and anthropogenic stressors will influence or even impair these mechanisms. We wish to measure these alterations using in-vivo non invasive MRI on carps and study the adaptation kinetics.

Researcher(s)

• Promoter: Van Der Linden Annemie

Research team(s)

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Van Der Linden Annemie

Research team(s)

Project type(s)

• Research Project

Abstract

Within this project we aim to develop an in-vivo non invasive method which allows to determine blood perfusion in tissues using Magnetic Resonance Imaging techniques. The ultimate goal is to implement these techniques to realise blood pefusion measurements in the rat brain and to measure tumor perfusion in a tumor animal model in nude mice.

Researcher(s)

• Promoter: Dommisse Roger
• Co-promoter: Van Der Linden Annemie

Research team(s)

Project type(s)

• Research Project

Abstract

In-vivo Magnetic Resonance Imaging en Spectroscopy enables one to obtain information on biochemical and physiological processes in a non-invasive and in-vivo manner. In the framework of this project we will have access to an MR microscope which allows to perform this type of research with a very high spatial and spectral resolution on small animals.

Researcher(s)

• Promoter: Van Der Linden Annemie

Research team(s)

Project type(s)

• Research Project

Abstract

With NMR 'flow imaging' techniques it is possible to measure flow speed of laminary flows. One can also measure the complete course of speed of pulsed flow (mechanical hydraulic model of arterial blood flow) provided that an adapted triggering is applied. A recently self-built gradient set will allow to obtain a higher image resolution.

Researcher(s)

• Promoter: Raman Erik
• Co-promoter: Van Camp Karel
• Co-promoter: Van Der Linden Annemie

Research team(s)

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Vanderborght Oscar L J
• Co-promoter: Blust Ronny
• Co-promoter: Van Der Linden Annemie

Research team(s)

Project type(s)

• Research Project

Abstract

The effectiveness of a newly developed cerebroprotective compound will be evaluated by in-vivo T2- and diffusion-weighted NMR imaging of treated and untreated rats suffering form focal cerebral ischemic lesions in the brain cortex.

Researcher(s)

• Promoter: Van Der Linden Annemie

Research team(s)

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Van Der Linden Annemie
• Co-promoter: Dommisse Roger

Research team(s)

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Van Der Linden Annemie
• Fellow: Kloeck Chantal

Research team(s)

Project type(s)

• Research Project

Abstract

The energy status can be considered as a measure for the physiological fitness of an organism. 31P NMR spectroscopy allows to determine the energy status of a testorganism (carp, mussel) submitted to stress (hypoxia, anoxia) and this in an non-invasive and in vivo manner. Chemical stressors (PCB, heavy metals...) will have a measurable influence on the energy status of the test animal and this influence will in its turn function as a measure for the severity of the environmental stress.

Researcher(s)

• Promoter: Dommisse Roger
• Co-promoter: Blust Ronny
• Co-promoter: Decleir Walter
• Co-promoter: Van Der Linden Annemie

Research team(s)

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Van Der Linden Annemie
• Fellow: Kloeck Chantal

Research team(s)

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Van Der Linden Annemie

Research team(s)

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Van Der Linden Annemie
• Fellow: Kloeck Chantal

Research team(s)

Project type(s)

• Research Project