Research Jean-Pierre Timmermans

Abstract

Mast cells are immune cells that are typically associated with allergic reactions at mucosal surfaces. Here, mast cells form operating units with sensory nerves and can contribute to sensations of itch and pain. In the context of Irritable Bowel Syndrome (IBS), a frequently occurring gastrointestinal disorder characterized by abnormal pain signaling (i.e. visceral hypersensitivity), the involvement of abnormal mast cell functioning has been recognized, but the exact receptors and signaling mechanisms driving this aberrant mast cell functioning remain poorly understood. In this respect, the presence of a novel IgE-independent, 'pseudo-allergic' pathway of mast cell activation pathway in the colon, consisting of mouse Mrgprb2 and its human counterpart MRGPRX2, was recently discovered our lab. In this PhD project, I will focus on the specific role of this novel Mrgprb2/X2-mediated signaling pathway as a driver of aberrant mast cell functioning in the pathophysiology of IBS and associated visceral hypersensitivity. In this way, my PhD project might generate a novel paradigm in our understanding of IBS pathophysiology and may form a solid foundation for further studies into the therapeutic potential of this pathway in these conditions.

Researcher(s)

• Promoter: Timmermans Jean-Pierre
• Co-promoter: Ibiza Martinez Sales
• Fellow: Lambeets Lana

Research team(s)

• Laboratory of cell biology and histology

Project type(s)

• Research Project

Abstract

The Antwerp Centre for Advanced Microscopy (ACAM) provides high-end service for visualizing biological samples from the nano- to the mesoscale. Its mission is to be the go-to hub for demanding microscopy-oriented work and to exploit its quantitative imaging expertise to foster research excellence at the University of Antwerp. To do so, ACAM assists with project planning, sample preparation, microscope selection and use, image analysis and visualization, and data interpretation. ACAM manages 10 advanced microscope systems, a server for image data warehousing and several workstations for image analysis. High-dimensional imaging is a major focus with light sheet microscopy, ultrafast live cell imaging and high-throughput screening as flagship technologies. Next to novel hardware acquisition and maintenance, ACAM develops its own software algorithms and evaluates experimental accessory setups. Routine training and thematic courses are organized to assure apt knowledge transfer regarding new technologies, optimal equipment usage and experimental reproducibility. ACAM pursues an open science policy and invests in making its data adhere to FAIR data principles. By combining breadth and depth in offered technology, and by keeping the pulse of the rapidly developing imaging field, ACAM aims at empowering researchers to perform science with high impact.

Researcher(s)

• Promoter: De Vos Winnok
• Promoter: Timmermans Jean-Pierre
• Co-promoter: De Vos Winnok
• Co-promoter: Kumar-Singh Samir
• Co-promoter: Pintelon Isabel
• Co-promoter: Timmermans Jean-Pierre

Research team(s)

• Laboratory of cell biology and histology

Project website

Project type(s)

• Research Project

Abstract

IMARK capitalizes on the deeply rooted expertise in biomedical imaging at the University of Antwerp to push the boundaries of precision medicine. By resolving molecular and structural patterns in space and time, IMARK aims at expediting biomarker discovery and development. To this end, it unites research groups with complementary knowledge and tools that cover all aspects of imaging-centred fundamental research, preclinical validation and clinical evaluation. IMARK harbours high-end infrastructure for electron and light microscopy, mass spectrometry imaging, magnetic resonance imaging, computed tomography, positron emission tomography and single-photon emission computed tomography. Moreover, IMARK members actively develop correlative approaches that involve multiple imaging modalities to enrich information content, and conceive dedicated image analysis pipelines to obtain robust, quantitative readouts. This unique blend of technologies places IMARK in an excellent position as preferential partner for public-private collaborations and offers strategic advantage for expanding the flourishing IP portfolio. The major application fields of the consortium are neuroscience and oncology. With partners from the Antwerp University Hospital and University Psychiatric Centre Duffel, there is direct access to patient data/samples and potential for translational studies.

Researcher(s)

• Promoter: De Vos Winnok
• Co-promoter: Baets Jonathan
• Co-promoter: Baggerman Geert
• Co-promoter: Bertoglio Daniele
• Co-promoter: Bogers John-Paul
• Co-promoter: Coppens Violette
• Co-promoter: Elvas Filipe
• Co-promoter: Keliris Georgios A.
• Co-promoter: Kumar-Singh Samir
• Co-promoter: Mertens Inge
• Co-promoter: Morrens Manuel
• Co-promoter: Staelens Steven
• Co-promoter: Stroobants Sigrid
• Co-promoter: Timmerman Vincent
• Co-promoter: Timmermans Jean-Pierre
• Co-promoter: Verhaeghe Jeroen
• Co-promoter: Verhoye Marleen
• Fellow: Lanens Dirk
• Fellow: Prasad Aparna

Research team(s)

• Laboratory of cell biology and histology

Project type(s)

• Research Project

Abstract

Neuropathological research is an interdisciplinary field, in which imaging and image-guided interventions have become indispensable. However, the rapid proliferation of ever-more inquisitive technologies and the different scales at which they operate have created a bottleneck at the level of integration, a) of the diverse image data sets, and b) of multimodal image information with omics-based and clinical repositories. To meet a growing demand for holistic interpretation of multi-scale (molecule, cell, organ(oid), organism) and multi-layered (imaging, omics, chemo-physical) information on (dys)function of the central and peripheral nervous system, we have conceived μNEURO, a consortium comprising eight established teams with complementary expertise in neurology, biomedical and microscopic imaging, electrophysiology, functional genomics and advanced data analysis. The goal of μNEURO is to expedite neuropathological research and identify pathogenic mechanisms in neurodevelopmental and -degenerative disorders (e.g., Alzheimer's Disease, epilepsy, Charcot-Marie-Tooth disease) on a cell-to-organism wide scale. Processing large spatiotemporally resolved image data sets and cross-correlating multimodal images with targeted perturbations takes center stage. Furthermore, inclusion of (pre)clinical teams will accelerate translation to a clinical setting and allow scrutinizing clinical cases with animal and cellular models. As knowledge-hub for neuro-oriented image-omics, μNEURO will foster advances for the University and community including i) novel insights in molecular pathways of nervous system disorders; ii) novel tools and models that facilitate comprehensive experimentation and integrative analysis; iii) improved translational pipeline for discovery and validation of novel biomarkers and therapeutic compounds; iv) improved visibility, collaboration and international weight fueling competitive advantage for large multi-partner research projects.

Researcher(s)

• Promoter: Sijbers Jan
• Co-promoter: Baets Jonathan
• Co-promoter: Cras Patrick
• Co-promoter: De Vos Winnok
• Co-promoter: Giugliano Michele
• Co-promoter: Kumar-Singh Samir
• Co-promoter: Staelens Steven
• Co-promoter: Stroobants Sigrid
• Co-promoter: Timmerman Vincent
• Co-promoter: Timmermans Jean-Pierre
• Co-promoter: Verhoye Marleen
• Co-promoter: Weckhuysen Sarah

Research team(s)

• Vision lab

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Timmermans Jean-Pierre

Research team(s)

• Laboratory of cell biology and histology

Project type(s)

• Research Project

Abstract

Cryo-electron microscopy (cryo-EM) has evolved tremendously over the last five years, thereby becoming a promising method to gain high-resolution structural information on proteins with a relevance in human (patho)physiology (e.g., cancer, host-pathogen interactions, and neuropathologies). This rapid evolution has sparked the interest of pharmaceutical companies in cryo-EM, since obtaining detailed structural information on proteins yields better insights into their function, which can be used to develop novel and/or better pharmaceuticals. However, as a result of its success, several inefficiencies within the cryo-EM workflow have emerged, especially related to sample preparation. Novel technologies have been proposed to optimize these, but these new techniques (i) often address only a single step within the overall workflow, (ii) are incompatible with other novel protocol/procedures or (iii) are difficult to implement by non-expert users. In a previous PoC study we developed a novel type of affinity grid that can be used for on-grid protein purification. Furthermore, market interviews have revealed that the introduction of this technology is best achieved through a service for protein structure determination (including a workflow from protein sample to protein structure) rather than simply providing the technology as such. The aim of this follow-up PoC is to validate the technology by resolving different protein structures using this grid technology and meanwhile establishing a service pipeline for high-resolution protein structure determination. This will illustrate the value of the grids towards potential customers (Pharma, Biotech) and investors.

Researcher(s)

• Promoter: Timmermans Jean-Pierre
• Co-promoter: Sterckx Yann

Research team(s)

• Laboratory of cell biology and histology

Project type(s)

• Research Project

Abstract

Crucial insights in cell and developmental biology have been gained by virtue of live cell imaging technology. Along with a growing complexity of cellular models and the finesse with which they can be genetically engineered, comes a demand for more advanced microscopy. In brief, modern comprehensive cell systems research (cellomics) requires light-efficient, intelligent and interactive imaging modalities. To address this shared need, our consortium has identified a state-of-the art platform that allows ultrafast, yet minimally invasive imaging of small to medium-sized biological samples (from single cells to organoids) at high resolution, so as to capture dynamic events that range in timescale from voltage fluctuations to successive cell divisions. To only focus on those events that are truly of interest, and thereby boost throughput, the system is equipped with online image recognition capabilities. Finally, to allow targeted perturbations such as local damage induction or optogenetic switching, small regions can be selectively illuminated in the field of view. With this level of control, it will become possible to interrogate (sub-)cellular processes with unprecedented detail. The platform readily finds applications in diverse frontline research fields including neuroscience, cardiovascular research and infectious diseases, rendering it an indispensable asset for the applicants, the microscopy core facility and the University of Antwerp.

Researcher(s)

• Promoter: De Vos Winnok
• Co-promoter: Caljon Guy
• Co-promoter: De Meyer Guido
• Co-promoter: Jordanova Albena
• Co-promoter: Rademakers Rosa
• Co-promoter: Timmerman Vincent
• Co-promoter: Timmermans Jean-Pierre
• Co-promoter: Vissenberg Kris
• Co-promoter: Weckhuysen Sarah

Research team(s)

• Laboratory of cell biology and histology

Project type(s)

• Research Project

Abstract

Digital pathology involves high-speed, high-resolution digital acquisition of images representing entire stained tissue sections from glass slides and allows them to be viewed directly in much the same way as standard microscopy. While this creates a permanent record of histological slide data and facilitates data sharing with collaborators, importantly, it allows analysis, quantification and objective pathological assessment of entire tissue samples, which is now current practice in pre-clinical and clinical research. We propose to acquire a high-resolution whole-slide scanner, notably absent at UA, not only to facilitate research at the promotors' groups, but virtually any research group performing basic, pre-clinical or clinical research at UA involving histopathology. We firmly believe that acquisition of such a digital scanner will help research groups at UA to stay competitive in biomedical research, facilitate and forge scientific and industrial collaborations at UA and beyond, and generate important industrial revenues.

Researcher(s)

• Promoter: Kumar-Singh Samir
• Co-promoter: Stroobants Sigrid
• Co-promoter: Timmermans Jean-Pierre
• Co-promoter: Vervaet Benjamin

Research team(s)

• Laboratory of cell biology and histology

Project type(s)

• Research Project

Abstract

The general purpose of this project is the development of an optimized prototype of the total lab-on-a-chip for cryo-EM, i.e. a cryo-EM affinity grid in combination with a microfluidic chip. The latter will enable the purification of bioparticles (i.e. proteins) which will then be loaded on a cryo-EM affinity grid.

Researcher(s)

• Promoter: Timmermans Jean-Pierre
• Fellow: Van Putte Wouter

Research team(s)

• Laboratory of cell biology and histology

Project type(s)

• Research Project

Abstract

Cryo-electron microscopy (cryo-EM) has evolved tremendously over the last five years, thereby becoming a promising method to gain high-resolution structural information on therapeutically relevant proteins involved in cancer, bacterial or viral infections, or neuropathologies. This rapid evolution has sparked the interest of pharmaceutical companies in cryo-EM, because protein structure information may yield better insights into the functioning of proteins, which can be used to develop better drugs. However, as a result of its success several inefficiencies within the cryo-EM workflow have emerged, especially related to sample preparation. Novel technologies have been proposed to optimize these, but these new techniques (i) often address only a single step within the overall workflow, (ii) are incompatible with other novel solutions or (iii) are difficult to implement by non-expert users. The objective of this PoC study is to revive affinity grids, electron microscopy grids that can be used for on-grid protein purification, and a potential solution to bypass some of the existing bottlenecks. by introducing novel recent developments in nanotechnology. The novel grid will be part of a larger international initiative aimed at developing an integrated workflow for highthroughput cryo-EM.

Researcher(s)

• Promoter: Timmermans Jean-Pierre
• Co-promoter: Van Putte Wouter

Research team(s)

• Laboratory of cell biology and histology

Project website

Project type(s)

• Research Project

Abstract

Developing novel neuroprotective and/or immune-modulating therapeutic strategies for almost every neurological disease or trauma requires, both for academia and pharmaceutical industry, the existence of robust in vitro cell culture models to mimic disease-associated pathological events. Unfortunately, a complex interplay between multiple central nervous system (CNS) cell types and multiple cell types from the body's peripheral immune system, cannot be easily recapitulated by currently used 2-dimensional (2D) co-culture assays. It is exactly therefore that successful pre-clinical experimental efficacy has proved to be very difficult to translate into clinical benefit, and as a consequence there is an increasing gap in knowledge and progress between bench and bed side. One highly promising novel approach to improve the predictive power of in vitro human neuro-immune research consists in developing modular 3D brain organoids that resemble brain tissue at the structural, cellular and functional level. Within this project we aim to develop and optimize a new method for generating isogenic 3D brain organoids, comprising human pluripotent stem cell (hPSC)-derived neurons, astrocytes and microglia. Furthermore, hPSC-derived astrocytes and endothelial cells will be used to create a blood-brain-barrier model for physical separation of hPSC-derived macrophages from the generated human 3D brain organoids. Together, this integrated cell system will represent a powerful new 3D human neuro-immune cell culture paradigm. Within this multidisciplinary IOF-SBO project, the methodological approach to generate 3D brain organoids, combined with the experience in the field of clinical research and the availability of patient samples, is truly unique and will - in first instance - highly contribute to the field of in vitro cerebrovascular disease modelling and treatment validation. Furthermore, our aims to install an integrated 3D brain organoid technology platform at the University of Antwerp, will - given the current scientific and economic interests – allow for both short-term and long-term valorisation of our combined efforts, with both intellectual (PhD-theses, A1 publications) as well as financial (contract research) revenues.

Researcher(s)

• Promoter: Ponsaerts Peter
• Co-promoter: De Vos Winnok
• Co-promoter: Jorens Philippe
• Co-promoter: Timmermans Jean-Pierre
• Co-promoter: Wouters An

Research team(s)

• Laboratory for Experimental Hematology (LEH)

Project type(s)

• Research Project

Abstract

Since a few years, evidence is growing that a disease process affecting the central nervous system (CNS) can also involve its enteric counterpart (ENS) and vice-versa. Indeed, various neurodegenerative disorders are accompanied or even preceded by gastrointestinal malfunctions. This relationship is well-documented for prion diseases and Parkinson's disease, but has not yet been scrutinized for Alzheimer's disease (AD), a devastating neurodegenerative disorder that is typified by a progressive and debilitating cognitive decline. A defining feature of AD is the accumulation of misfolded amyloid-beta peptides. Considering that the CNS and ENS are highly interconnected, the gut microbiome produces amyloids that can cross-seed polymerization, and inflammation promotes amyloid build-up, it is highly conceivable that the gut is a vulnerable node for amyloid-driven degeneration. Yet, the mechanisms underlying cellular processing of amyloids in the ENS are poorly characterized. Hence, with this research project, we will investigate the entry routes, spreading behaviour and cellular effects of microbial and host-derived amyloid proteins in the ENS. Using innovative imaging technologies and well-defined molecular characterization methods, this work will provide a solid basis for refining the gut-brain axis theory in the context of AD and will open novel avenues for both fundamental and clinical research with relevance for a broad range of proteopathic neurodegenerative diseases.

Researcher(s)

• Promoter: Timmermans Jean-Pierre
• Co-promoter: De Vos Winnok

Research team(s)

• Laboratory of cell biology and histology

Project type(s)

• Research Project

Abstract

Alzheimer's disease (AD) currently affects 1 in 9 individuals over 65 years of age but its prevalence will only rise against the background of a steadily greying population. With no cure currently available and diagnosis relying on the assessment of late-stage cognitive decline, it is imperative that novel early medical entry points are explored through original fundamental research. Recent insights suggest that the gut may be a vulnerable node for amyloid-driven neurodegeneration. That is why we want to define the origin, entry routes and spreading behaviour of amyloid proteins in the enteric nervous system (ENS). Using innovative imaging technologies and well-defined molecular analyses, we will shed light on a novel gut-brain relationship with relevance for future (pre-)clinical research. As the gut represents a unique, minimally invasive window to assess neuropathology, our work may spark the development of early biomarkers that directly report on disease progression in AD-patients. Moreover, confirming the notion that microbial-derived amyloids could represent a putative trigger for pathology may cause a paradigm shift for AD therapy."

Researcher(s)

• Promoter: Timmermans Jean-Pierre
• Co-promoter: De Vos Winnok

Research team(s)

• Laboratory of cell biology and histology

Project type(s)

• Research Project

Abstract

This project comprises the purchase of a new scanning electron microscope for studying surface features of biomedical samples and replaces the SEM515 (purchased in 1987). The new instrument allows examination of samples both at low and high vacuum conditions.

Researcher(s)

• Promoter: Timmermans Jean-Pierre

Research team(s)

• Laboratory of cell biology and histology

Project type(s)

• Research Project

Abstract

Mas-related G-Protein coupled receptors (Mrgpr's) have a putative role in sensory processes, including itch and pain. Previous studies on Mrgpr's in intestinal inflammatory conditions revealed marked expressional changes in them, specifically in sensory neurons of enteric nervous system (ENS). The fact that most of Mrgpr's are still classified as orphan, has hampered the study of their functions and regulatory mechanisms. This project develops novel approaches to explore the function of Mrgpr despite their current orphan status and explores protease-linked activation mechanisms of these Mrgprs. MrgprE and MrgprF are of special interest since these receptors are co-expressed in both enteric plexusses (myenteric and submucosal), and co-regulated in Schistosoma mansoni and TNBS-induced ileitis murine models. Mouse and human MrgprE and MrgprF are orthologous, and ongoing studies on gut biopsies in our laboratory confirm expression of MrgprE and MrgprF in the human gastrointestinal tract. This has prompted us to elucitade the interaction of human (h-) MrgprE and MrgprF. Therefore, we employed various biophysical and biochemical techniques including a novel luciferase complementation technique (NanoBiT), Bioluminescence resonance energy transfer (BRET), Fluorescence resonance energy transfer (FRET) and co-immunoprecipitation to validate our hypothesis. Results gave concrete evidence of h-MrgprE/h-MrgprF heteromerization, as well as h-MrgprE and h-MrgprF homomerization respectively. Over the last decade examples of GPCR's homo- and hetero-merizations are increasing at accelerating pace. Homo- and heteromeric states of GPCR's modulate the signal transductions capabilities and, therefore, regulate G-protein-dependent or -independent signalling associated with them. Nevertheless, biophysical tools used for detecting homo- and heteromerization of GPCR's account only for the oligomerization state but do not define their affinity and the competition among interacting partners for oligomerization. Therefore, there is need of novel protein-protein interaction (PPI) tools to ascertain affinity and competition among GPCR's. Starting from our obtained results, we are developing a quantitative NanoBiT assay, which will expand the current PPI tools and provide a better tool to study affinity, competition, biased agonism and transient protein interactions.

Researcher(s)

• Promoter: Timmermans Jean-Pierre
• Fellow: Arora Rohit

Research team(s)

• Laboratory of cell biology and histology

Project type(s)

• Research Project

Abstract

The application concerns an innovative microscopy platform for visualizing cells, tissue specimen and living small model organisms in three dimensions at unprecedented speed and with excellent resolution and contrast. As a unique feature, the platform is equipped with a light-sheet module, which is based on an orthogonal configuration of laser-generated, micrometer-thin plane illumination and sensitive one-shot detection. Seamless integration with confocal modalities enables imaging the same sample from the micro- to the mesoscale. The device has a broad application radius in the neurosciences domain inter alia for studying neurodegeneration and -regeneration (e.g. whole brain imaging, optogenetics); but it also has direct utility in various other fields such as cardiovascular research (e.g. plaque formation and stability), plant developmental research (e.g. protein localization during plant growth) and ecotoxicology (e.g. teratogenicity and developmental defects in zebrafish). Furthermore, its modular construction will enable adaptation and targeted expansion for future imaging needs.

Researcher(s)

• Promoter: Timmermans Jean-Pierre
• Co-promoter: Adriaensen Dirk
• Co-promoter: De Meyer Guido
• Co-promoter: De Vos Winnok
• Co-promoter: D'Haese Patrick
• Co-promoter: Giugliano Michele
• Co-promoter: Jordanova Albena
• Co-promoter: Keliris Georgios A.
• Co-promoter: Knapen Dries
• Co-promoter: Maudsley Stuart
• Co-promoter: Ponsaerts Peter
• Co-promoter: Timmerman Vincent
• Co-promoter: Vissenberg Kris

Research team(s)

• Laboratory of cell biology and histology

Project type(s)

• Research Project

Abstract

EGAMI stands for Expert Group Antwerp Molecular Imaging. Moreover, EGAMI is the mirror word of 'image'. EGAMI clusters the internationally recognized expertise in the profession of fundamental and biomedical imaging at the University of Antwerp: the Bio-Imaging Lab, the Molecular Imaging Center Antwerp (MICA), Radiology, the Laboratory for Cell Biology and Histology, and the Vision Lab (for post-processing of medical images). EGAMI's mission is providing an integrated research platform that comprises all aspects of multimodality translational medical imaging. Multimodality refers to the integration of information from the various imaging techniques. Within EGAMI, there is pre-clinical and clinical expertise and infrastructure for magnetic resonance imaging (MRI), computed tomography (CT), positron emission tomography (PET), and single-photon emission computed tomography (SPECT). EGAMI executes projects ranging from applied biomedical (imaging) and fundamental research to imaging methodologies. Die applied biomedical research focusses on the research fields neuro(bio)logy (i.e. development and validation of biomarkers (as well as therapy evaluation) for diseases like Alzheimer's, schizophrenia, multiple sclerosis etc.) and oncology (i.e. biomarkers for improved patient stratification and therapy monitoring). Since the pre-clinical biomedical research within EGAMI makes use of miniaturized versions of imaging equipment for humans (scanners) is it inherently translational, in other words initial findings acquired in animal experiments can be translated into clinical applications for improved diagnosis and treatment of patients ('from bench to bedside'). Beside the application of imaging in the biomedical research, EGAMI also conducts projects that aim to achieve an improvement and optimization of the imaging methodology. The expertise of the MICA (e.g. the development of new radiotracers) and of the Vision Lab (e.g. the development of image reconstruction, segmentation, and analysis algorithms) offers here the strategic platform to assemble intellectual property rights.

Researcher(s)

• Promoter: Van Der Linden Annemie
• Co-promoter: Parizel Paul
• Co-promoter: Sijbers Jan
• Co-promoter: Staelens Steven
• Co-promoter: Timmermans Jean-Pierre
• Fellow: Guns Pieter-Jan
• Fellow: Lanens Dirk
• Fellow: Van Putte Wouter

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

This project represents a formal research agreement between UA and on the other hand VITO. UA provides VITO research results mentioned in the title of the project under the conditions as stipulated in this contract.

Researcher(s)

• Promoter: Timmermans Jean-Pierre

Research team(s)

Project type(s)

• Research Project

Abstract

The mission of the Centre for R&D Monitoring (ECOOM) is to develop and consolidate a consistent and performing system of R&D and Innovation (RD&I) indicators which provides the Flemish Governement with actual and relevant statistic data for mapping and monitoring the RD&I efforts in the Flemish region. At the same time ECOOM develops a portfolio of relevant scientific research activities to support this mission. Therefore, ECOOM (1) has a series of targeted, long term task packages to map, measure and analyze the Flemish R&D and innovation potential; (2) ensures that a data management infrastructure is available to the Flemish Government containing bibliometric and technometric data and data on innovation and PhDs; (3) provides qualified staffing; (4) builds the necessary and appropriate IT-infrastructure and (5) is able to conduct ad-hoc task or projects at request of the Flemish Government. ECOOM acts as an inter-association center.

Researcher(s)

• Promoter: Engels Tim
• Co-promoter: Blust Ronny
• Co-promoter: Timmermans Jean-Pierre

Research team(s)

Project website

Project type(s)

• Research Project

Abstract

The current project aims to further explore the role and regulation of Mrgs in the gastrointestinal tract. We will study the function of Mrg in neurons and mast cells using advanced microscopical and cell biological techniques. Furthermore we plan to explore the role of Mrg beyond inflammatory intestinal pathologies, i.e. to investigate the involvement of these receptors in irritable bowel syndrome. We will combine animal models with research in human tissues to study the translational potential of the findings. Ultimately, this study aims to provide insight in the role of Mrg receptors in gastrointestinal pathologies.

Researcher(s)

• Promoter: Timmermans Jean-Pierre
• Co-promoter: Buckinx Roeland

Research team(s)

• Laboratory of cell biology and histology

Project type(s)

• Research Project

Abstract

This project represents a formal research agreement between UA and on the other hand private institution. UA provides private institution research results mentioned in the title of the project under the conditions as stipulated in this contract.

Researcher(s)

• Promoter: Timmermans Jean-Pierre

Research team(s)

• Laboratory of cell biology and histology

Project type(s)

• Research Project

Abstract

The funds are allocated for activities relating to the support and supervision of young researchers. The grant will be used to create a framework for and the development, implementation and strengthening of activities related to the following objectives: Training of young researchers; Career development and promotion of career prospects of young researchers; Strengthening the international orientation in the careers of young researchers."

Researcher(s)

• Promoter: Timmermans Jean-Pierre

Research team(s)

Project type(s)

• Research Project

Abstract

The current project will follow the development of neuroinflammation together with functional brain integrity and behavioural outcome in a rodent model of maternal immune activation in vivo utilising state-of-the-art multimodal imaging biomarkers.This project will generate highly novel information about the contribution of neuroinflammation to the development of schizophrenia and its consequences for the functional integrity of the brain, and eventually provide a rationale for the implementation of novel disease-modifying strategies.

Researcher(s)

• Promoter: Dedeurwaerdere Stefanie
• Co-promoter: Staelens Steven
• Co-promoter: Timmermans Jean-Pierre
• Co-promoter: Van Der Linden Annemie

Research team(s)

• Translational Neurosciences (TNW)

Project type(s)

• Research Project

Abstract

In this study, we will investigate the role of autophagy (a cell survival and death pathway) and adiponectin (an endogenous hormone produced by fat cells) in a protective post-myocardial infarction reperfusion therapy called postconditioning. Adiponectin has protective myocardial effects that limit lethal reperfusion injury. However, patients with central obesity have low plasma levels of adiponectin, which may confound the cardioprotective properties of postconditioning.

Researcher(s)

• Promoter: Vrints Christiaan
• Co-promoter: Claeys Marc
• Co-promoter: De Meyer Guido
• Co-promoter: Martinet Wim
• Co-promoter: Timmermans Jean-Pierre

Research team(s)

Project type(s)

• Research Project

Abstract

This project aims at identifying pathways that are involved in the normal function of NEBs in postnatal lungs. The NEB microenvironment will be dissected in large numbers via advanced laser capture microdissection and will be used for gene expression analysis.

Researcher(s)

• Promoter: Adriaensen Dirk
• Co-promoter: Timmermans Jean-Pierre

Research team(s)

• Laboratory of cell biology and histology

Project type(s)

• Research Project

Abstract

This project represents a research contract awarded by the University of Antwerp. The supervisor provides the Antwerp University research mentioned in the title of the project under the conditions stipulated by the university.

Researcher(s)

• Promoter: Van Der Linden Annemie
• Co-promoter: Adriaensen Dirk
• Co-promoter: Timmermans Jean-Pierre
• Fellow: De Visscher Geofrey
• Fellow: Guns Pieter-Jan

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

This project represents a research contract awarded by the University of Antwerp. The supervisor provides the Antwerp University research mentioned in the title of the project under the conditions stipulated by the university.

Researcher(s)

• Promoter: Timmermans Jean-Pierre

Research team(s)

Project type(s)

• Research Project

Abstract

This project represents a research agreement between the UA and on the onther hand IWT. UA provides IWT research results mentioned in the title of the project under the conditions as stipulated in this contract.

Researcher(s)

• Promoter: Del-Favero Jurgen
• Co-promoter: Timmermans Jean-Pierre

Research team(s)

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Timmermans Jean-Pierre

Research team(s)

• Laboratory of cell biology and histology

Project type(s)

• Research Project

Abstract

This project represents a formal research agreement between UA and on the other hand the Flemish Public Service. UA provides the Flemish Public Service research results mentioned in the title of the project under the conditions as stipulated in this contract.

Researcher(s)

• Promoter: Timmermans Jean-Pierre
• Fellow: Verstraelen Peter

Research team(s)

• Laboratory of cell biology and histology

Project type(s)

• Research Project

Abstract

This project proposal aims at verifying in which way the different homing signals originating from ischemic tissue lead endothelial progenitor cells - via induction of the PI3K-Akt-eNOS pathway - to express cellular adhesion molecules, which results in efficient homing, adhesion and diapedesis.

Researcher(s)

• Promoter: Vrints Christiaan
• Co-promoter: Timmermans Jean-Pierre
• Fellow: Everaert Bert

Research team(s)

Project type(s)

• Research Project

Abstract

The mission of the Centre for R&D Monitoring (ECOOM) is to develop and consolidate a consistent and performing system of R&D and Innovation (RD&I) indicators which provides the Flemish Governement with actual and relevant statistic data for mapping and monitoring the RD&I efforts in the Flemish region. At the same time ECOOM develops a portfolio of relevant scientific research activities to support this mission. Therefore, ECOOM (1) has a series of targeted, long term task packages to map, measure and analyze the Flemish R&D and innovation potential; (2) ensures that a data management infrastructure is available to the Flemish Government containing bibliometric and technometric data and data on innovation and PhDs; (3) provides qualified staffing; (4) builds the necessary and appropriate IT-infrastructure and (5) is able to conduct ad-hoc task or projects at request of the Flemish Government. ECOOM acts as an inter-association center.

Researcher(s)

• Promoter: Timmermans Jean-Pierre

Research team(s)

Project type(s)

• Research Project

Abstract

This project represents a research contract awarded by the University of Antwerp. The supervisor provides the Antwerp University research mentioned in the title of the project under the conditions stipulated by the university.

Researcher(s)

• Promoter: Timmermans Jean-Pierre
• Co-promoter: De Groote Chantal
• Co-promoter: De Meyer Guido
• Co-promoter: Timmerman Vincent
• Co-promoter: Van Marck Eric
• Co-promoter: Verbelen Jean-Pierre

Research team(s)

• Laboratory of cell biology and histology

Project type(s)

• Research Project

Abstract

This project represents a formal research agreement between UA and on the other hand Francqui-fonds. UA provides Francqui-fonds research results mentioned in the title of the project under the conditions as stipulated in this contract.

Researcher(s)

• Promoter: Timmermans Jean-Pierre
• Fellow: Giugliano Michele

Research team(s)

Project type(s)

• Research Project

Abstract

The aim of this project is (1) to verify whether CRF, UCN2 and UCN3, in addition to UCN1 and CST, also have anti-inflammatory effects on intestinal inflammation; (2) to determine, on the one hand, the effect of these neuropeptides on the neuroimmune interaction during inflammation and, on the other hand, the importance of this effect in the therapeutical effects of these neuropeptides; and (3) to examine which receptors are effectively involved in this process.

Researcher(s)

• Promoter: Timmermans Jean-Pierre
• Co-promoter: Van Nassauw Luc

Research team(s)

• Laboratory of cell biology and histology

Project website

Project type(s)

• Research Project

Abstract

In this project animal models were used to study the interaction between neuronal components of the GI tract and elements of the immune system, more particularly mast cells and dendritic cells, during inflammation. This interaction appears to play an important role in the inflammatory response and might offer a better insight for the observed hyperexcitability of afferent components of the enteric nervous system during gastrointestinal inflammation. This project will primarily focus on the possible role of cortistatin and urocortines in this interaction.

Researcher(s)

• Promoter: Timmermans Jean-Pierre
• Co-promoter: Adriaensen Dirk

Research team(s)

• Laboratory of cell biology and histology

Project type(s)

• Research Project

Abstract

1.Identification and topographical localisation of somatostatin receptors in the enteric nervous system of the non-inflamed mouse ileum using immunohistochemistry and reverse transcriptase-PCR. The somatostatin receptor-containing neurons are neurochemically characterised by means of multiple immunofluerescence stainings. 2.Comparison of the identity and expression pattern of somatostatin receptors in enteric neurons in 8-week Schistosoma mansoni-infected mice with non-inflamed ileum, using Real Time RT-PCR. 3.Optimisation of the protocol for isolation of mucosal mast cells from the lamina propria of 8-week Schistosoma mansoni-infected mice. 4.Identification and quantification of somatostatin receptors on in vitro mucosal mast cells and on mucosal mast cells isolated from the lamina propria of 8-week infected mice by means of immunohistochemistry, reverse transcriptase-PCR and Real Time RT-PCR. These results are substantiated in vivo by immunofluorescent detection of somatostatin receptors on mucosal mast cells in the gastrointestinal tract of Schistosoma mansoni-infected mice. 5.Study of a possible compensatory effect of somatostatin receptor type 2 deficiency on the identity and expression level of the somatostatin receptors in enteric neurons and musocal mast cells using immunohistochemistry, reverse transcriptase PCR and Real Time RT-PCR. 6.Study of the effect of administration of SOM and SOM analogues on the synthesis and secretion of inflammatory mediators by mucosal mast cells using cDNA microarray and ELISA. 7.Functional study of the effect of somatostatin on the neuroimmune interaction between mast cells and enteric neurons using mucosal mast cells and enteric neurons isolated from the gastrointestinal tract of Schistosoma mansoni-infected mice. Life cell imaging and multi-photon microscopy are applied to investigate the activity of both cell types after SOM stimulation.

Researcher(s)

• Promoter: Timmermans Jean-Pierre
• Fellow: Van Op den bosch Joeri

Research team(s)

• Laboratory of cell biology and histology

Project type(s)

• Research Project

Abstract

This project proposal aims at verifying in which way the different homing signals originating from ischemic tissue lead endothelial progenitor cells - via induction of the PI3K-Akt-eNOS pathway - to express cellular adhesion molecules, which results in efficient homing, adhesion and diapedesis.

Researcher(s)

• Promoter: Vrints Christiaan
• Co-promoter: Timmermans Jean-Pierre
• Fellow: Everaert Bert

Research team(s)

Project website

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Vrints Christiaan
• Co-promoter: Timmermans Jean-Pierre
• Fellow: Everaert Bert

Research team(s)

Project type(s)

• Research Project

Abstract

This project represents a research contract awarded by the University of Antwerp. The supervisor provides the Antwerp University research mentioned in the title of the project under the conditions stipulated by the university.

Researcher(s)

• Promoter: Timmermans Jean-Pierre

Research team(s)

• Laboratory of cell biology and histology

Project type(s)

• Research Project

Abstract

Specific aims of the project : 1) Detection of silent Kv subunits in the heart and their role in the slowly inactivating delayed rectifier current. 2) Transgenic analysis of the physiological role of silent subunits. 3) Do KChIP and KVI.5 interact stably and what are the functional consequences ? 4) What are the molecular determinants of the KChIP interaction with Kv1.5 subunits?

Researcher(s)

• Promoter: Snyders Dirk
• Co-promoter: Timmermans Jean-Pierre

Research team(s)

Project type(s)

• Research Project

Abstract

In normal liver, intrahepatic resistance changes with variations in portal blood flow, thereby keeping porta )ressure within normal limits. In cirrhosis however, both intrahepatic resistance and splanchnic blood flow are ncreased, resulting in portal hypertension. Although structural changes contribute most to the increasec ntrahepatic vascular resistance, it has become clear that not only fixed, but also dynamic factors add significantly the increased resistance. This dynamic part is caused by active contraction of septal and/or portal nyofibroblasts, activated hepatic stellate cells (HSC) and portal venules. Activated HSC contract in response tc rarious vasoactive substances and the cells are currently regarded as target cells for treating porta lypertension. Recent years have seen considerable progress towards understanding the signaling mechanism egulating non-muscle cell contractility. Whereas contraction of smooth muscle cells is governed primarily by Ca2+ signaling contractility of non-muscle cells is principally mediated by Rho signaling. It is not known as yet which of both mechanisms plays a greater role in HSC contractility. However, it has been speculated that, as HSC arE Ictivated and exhibit smooth muscle-like features, Ca2+ signaling becomes more important.

Researcher(s)

• Promoter: Timmermans Jean-Pierre

Research team(s)

• Laboratory of cell biology and histology

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Vrints Christiaan
• Co-promoter: Timmermans Jean-Pierre

Research team(s)

Project type(s)

• Research Project

Abstract

1.Identification and topographical localisation of somatostatin receptors in the enteric nervous system of the non-inflamed mouse ileum using immunohistochemistry and reverse transcriptase-PCR. The somatostatin receptor-containing neurons are neurochemically characterised by means of multiple immunofluerescence stainings. 2.Comparison of the identity and expression pattern of somatostatin receptors in enteric neurons in 8-week Schistosoma mansoni-infected mice with non-inflamed ileum, using Real Time RT-PCR. 3.Optimisation of the protocol for isolation of mucosal mast cells from the lamina propria of 8-week Schistosoma mansoni-infected mice. 4.Identification and quantification of somatostatin receptors on in vitro mucosal mast cells and on mucosal mast cells isolated from the lamina propria of 8-week infected mice by means of immunohistochemistry, reverse transcriptase-PCR and Real Time RT-PCR. These results are substantiated in vivo by immunofluorescent detection of somatostatin receptors on mucosal mast cells in the gastrointestinal tract of Schistosoma mansoni-infected mice. 5.Study of a possible compensatory effect of somatostatin receptor type 2 deficiency on the identity and expression level of the somatostatin receptors in enteric neurons and musocal mast cells using immunohistochemistry, reverse transcriptase PCR and Real Time RT-PCR. 6.Study of the effect of administration of SOM and SOM analogues on the synthesis and secretion of inflammatory mediators by mucosal mast cells using cDNA microarray and ELISA. 7.Functional study of the effect of somatostatin on the neuroimmune interaction between mast cells and enteric neurons using mucosal mast cells and enteric neurons isolated from the gastrointestinal tract of Schistosoma mansoni-infected mice. Life cell imaging and multi-photon microscopy are applied to investigate the activity of both cell types after SOM stimulation.

Researcher(s)

• Promoter: Timmermans Jean-Pierre
• Fellow: Van Op den bosch Joeri

Research team(s)

• Laboratory of cell biology and histology

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Timmermans Jean-Pierre

Research team(s)

• Laboratory of cell biology and histology

Project type(s)

• Research Project

Abstract

The aim of this study is the morphological, electrophysiological and neuropharmacological identification of the role, the mechanisms and the effects of the bi-directional interactions between the enteric nervous system and the enteric immune system on the functioning of the gastrointestinal tract under normal and inflammatory conditions.

Researcher(s)

• Promoter: Timmermans Jean-Pierre
• Fellow: Kroese Alfons

Research team(s)

• Laboratory of cell biology and histology

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Timmermans Jean-Pierre

Research team(s)

• Laboratory of cell biology and histology

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Timmermans Jean-Pierre

Research team(s)

• Laboratory of cell biology and histology

Project type(s)

• Research Project

Abstract

This project combines the technical expertise of the 3 research groups to study 3 topics: the cerebellum, enteric nervous system and potassium channels. Specifically we will study: classification of neurons in the granular layer, potassium channels in Purkinje cells and their inhibition of the deep nuclei; expression of potassium channels in viscerosensitive neurons and changes due to inflammatory mediators released by mast cells; and expression and function in the brain of 5 new potassium channel subunits.

Researcher(s)

• Promoter: De Schutter Erik
• Co-promoter: Snyders Dirk
• Co-promoter: Timmermans Jean-Pierre

Research team(s)

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Timmermans Jean-Pierre

Research team(s)

• Laboratory of cell biology and histology

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Timmermans Jean-Pierre
• Co-promoter: Van Marck Eric

Research team(s)

• Laboratory of cell biology and histology

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Timmermans Jean-Pierre

Research team(s)

• Laboratory of cell biology and histology

Project type(s)

• Research Project

Abstract

PARs are one of the possible sensory oeuron-specific molecular targets that can be useful for therapeutic treatment in inflammatory bowel disorders and gastrointestinal pain. This project aims at elucidating the role{s} of these receptors in the neuroimmune interactions between mast cells and the afferent component of the enteric nervous system in our schistosomiasis inflammation model.

Researcher(s)

• Promoter: Van Meir Frans
• Co-promoter: Timmermans Jean-Pierre

Research team(s)

• Laboratory of cell biology and histology

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Timmermans Jean-Pierre

Research team(s)

• Laboratory of cell biology and histology

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Timmermans Jean-Pierre

Research team(s)

• Laboratory of cell biology and histology

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Timmermans Jean-Pierre
• Fellow: De Jonge Frederik

Research team(s)

• Laboratory of cell biology and histology

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Timmermans Jean-Pierre
• Co-promoter: Van Nassauw Luc

Research team(s)

• Laboratory of cell biology and histology

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Blust Ronny
• Co-promoter: Timmermans Jean-Pierre

Research team(s)

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Schryvers Nick
• Co-promoter: Timmermans Jean-Pierre

Research team(s)

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Timmermans Jean-Pierre

Research team(s)

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Timmermans Jean-Pierre

Research team(s)

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Timmermans Jean-Pierre

Research team(s)

• Laboratory of cell biology and histology

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Timmermans Jean-Pierre

Research team(s)

• Laboratory of cell biology and histology

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Timmermans Jean-Pierre

Research team(s)

• Laboratory of cell biology and histology

Project type(s)

• Research Project

Abstract

The aim of this study is to morphologically and electrophysiologically characterize the intrinsic sensory neurons by means of multi-site optical recording after chemical or mechanostimulation.

Researcher(s)

• Promoter: Timmermans Jean-Pierre

Research team(s)

Project type(s)

• Research Project

Abstract

Within the morphological part of this project, the cellular localization and expression pattern of the DFNA5 gene product will be investigated by means of immunocytochemical techniques.

Researcher(s)

• Promoter: Timmermans Jean-Pierre

Research team(s)

• Laboratory of cell biology and histology

Project type(s)

• Research Project

Abstract

The main aims of this project are the study of 1. the correlation between morphological and physiological features of intrinsic esophageal neurons and 2. the role of interstitial cells of Cajal in this part of the gastrointestinal tract.

Researcher(s)

• Promoter: Timmermans Jean-Pierre
• Co-promoter: Adriaensen Dirk

Research team(s)

• Laboratory of cell biology and histology

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Timmermans Jean-Pierre
• Fellow: De Jonge Frederik

Research team(s)

Project type(s)

• Research Project

Abstract

Corticocerebellar Golgi cells are characterized as granule cell layer interneurones with large rounded or polygonal cell bodies and radiating dendritic arborizations. The present study will focus on Golgi cells in order to provide a more detailled morphological description, including their different synaptic inputs. In addition, Golgi cell morphology will be related to the whole of the cerebellar cortex.

Researcher(s)

• Promoter: Timmermans Jean-Pierre
• Fellow: Geurts Frederik

Research team(s)

Project type(s)

• Research Project

Abstract

Researcher(s)

• Promoter: Timmermans Jean-Pierre

Research team(s)

Project type(s)

• Research Project

Abstract

Complementary to the classical intracellular recording technique, two-photon microscopy and specific Ca++-indicators and/or membrane potential-sensitive fluorescente substances will be used in order to visualize neuronal activity within enteric neurocircuits following application of mechanical, electrical and chemical stimuli.

Researcher(s)

• Promoter: Timmermans Jean-Pierre

Research team(s)

Project type(s)

• Research Project

Abstract

Notwithstanding the simple and regular anatomical organisation of the cerebellum, its function and activity remains unclear. This project aims at better understanding the function and activity of the cerebellum applying computer simulations and experiments in both men and animals. To that end morphological and electrophysiological techniques will be used on rats and functional magnetic resonance imaging will be performed on rat and men.

Researcher(s)

• Promoter: Van Der Linden Annemie
• Co-promoter: Timmermans Jean-Pierre

Research team(s)

• Bio-Imaging lab

Project type(s)

• Research Project

Abstract

By means of visualization and recordings of membrane potential and/or intracellular Ca2+-changes, it will be attempted to analyse the spread of excitability in enteric neurons and to assess the differential activity of functional enteric neuron types in response to specific stimuli

Researcher(s)

• Promoter: Timmermans Jean-Pierre

Research team(s)

Project type(s)

• Research Project

Abstract

RAFO funding for the purchase of a knife maker to make glass knives to be used for making ultrathin sections with the ultramicrotome.

Researcher(s)

• Promoter: Timmermans Jean-Pierre

Research team(s)

Project type(s)

• Research Project

Abstract

This study has a dual aim: 1. Identification and analysis of intrinsic pathways in the porcine and human enteric nervous system using carbocyanine tracing. 2. Development of a dissociation technique for enteric neurons in large domestic animals in order to study the electrophysiological properties of various functional DiI-identified neuron classes.

Researcher(s)

• Promoter: Timmermans Jean-Pierre

Research team(s)

Project type(s)

• Research Project

Abstract

Some aspects of the research themes of the participating groups are studied in collaboration, using a multi-disciplinary approach made possible by combining the know-how of the different groups. The central theme is regulation-mechanisms in the gastrointestinal tract. Specific goals are: a) NANC-neurotransmission, especially NO- and VIP-mediated; b) mechanism of action of motilin and motilides; and c) the interaction between the enteric nervous system and inflammatory mediators.

Researcher(s)

• Promoter: Timmermans Jean-Pierre

Research team(s)

• Laboratory of cell biology and histology

Project type(s)

• Research Project

Abstract

We will study the relation between neuronal morphology and physiological function at several levels of complexity and in two different neuronal systems (the cerebellum and the enteric system). We want to determine the importance of single cell morphology for the classification of different neurons and its effects on second messenger metabolism, on the firing behavior of a particular neuron type, and at the network level. The reconstruction of the neuronal morphologies will be carried out jointly by the two research groups, while the applied physiological research methods are dependent on the neural systems under investigation.

Researcher(s)

• Promoter: De Schutter Erik
• Co-promoter: Timmermans Jean-Pierre

Research team(s)

Project type(s)

• Research Project

Abstract

Using a recent tracer technique within organotypic cultures, combined with immunocytochemical and in situ hybridization methods, the effects of schistosomiasis on the intrinsic component of the enteric nervous system, i.e. on the course of intrinsic nerve tracts and on the neurochemical characteristics of neurons, and also on the expression of cyto- and chemokines in non-neuronal cells, will be morphologically analyzed.

Researcher(s)

• Promoter: Van Nassauw Luc
• Co-promoter: Timmermans Jean-Pierre

Research team(s)

Project type(s)

• Research Project

Abstract

Corticocerebellar Golgi cells are characterized as granule cell layer interneurones with large rounded or polygonal cell bodies and radiating dendritic arborizations. The present study will focus on Golgi cells in order to provide a more detailled morphological description, including their different synaptic inputs. In addition, Golgi cell morphology will be related to the whole of the cerebellar cortex.

Researcher(s)

• Promoter: Timmermans Jean-Pierre
• Fellow: Geurts Frederik

Research team(s)

Project type(s)

• Research Project

Abstract

Immunohistochemical and in situ hybridisation study of the enteric innervation of the mouse under normal and inflammatory conditions.

Researcher(s)

• Promoter: Timmermans Jean-Pierre
• Fellow: Van Nassauw Luc

Research team(s)

Project type(s)

• Research Project

Abstract

RAFO co-funding for the purchase of an ultramicrotome with freeze unit to trim tissues and to prepare ultrathin sections and ultrathin frozen sections for TEM study, and semithin sections and semithin frozen sections for light microscopic research.

Researcher(s)

• Promoter: Timmermans Jean-Pierre

Research team(s)

Project type(s)

• Research Project

Abstract

Using so-called "fluopeptides" this study not only tries to determine the distribution pattern of several neuropeptides-receptors in the gastro-intestinal tract, but also to study the kinetics of these receptors under well-defined circumstances.

Researcher(s)

• Promoter: Adriaensen Dirk
• Co-promoter: Timmermans Jean-Pierre

Research team(s)

Project type(s)

• Research Project

Abstract

After identifying the sequence of motilin antibodies will be prepared in the rabbit to map motilin-containing cells by immunocytochemistry. Apart from the topographical localisation, the neurochemical features of neuron populations expressing motilin binding sites in the central nervous system will be determined. In addition, central effects will be studied by means of functional MRI after intraventricular injections of motilin and motilides.

Researcher(s)

• Promoter: Timmermans Jean-Pierre

Research team(s)

Project type(s)

• Research Project

Abstract

Using a combined method of neuronal tracing and immunocytochemistry, it is attempted to analyse the topographical ditribution and neurochemical coding of distinct enteric neuron populations in the digestive tract of large mammals

Researcher(s)

• Promoter: Timmermans Jean-Pierre

Research team(s)

Project type(s)

• Research Project

Abstract

This network aims at investigating the neuroendocrine interactions in various segments of the gastrointestinal tract from a functional-morphological and pharmacological point of view. In a first phase, the enteric nervous system and the relation to the specific targets (enterocytes, endocrine cells, neurons, muscle fibres) will be focused on. The task of this laboratory comprises the neurochemical and electrophysiological characterization of the different enteric neuron populations.

Researcher(s)

• Promoter: Timmermans Jean-Pierre

Research team(s)

Project type(s)

• Research Project

Abstract

Using non-radioactive labeled mRNA- or oligonucleotideprobes, in situ hybridization is applied to gain a better insight in the panel of bio-active substances that can be produced by the neuroepithelial endocrine cells in the respiratory system of vertebrates.

Researcher(s)

• Promoter: Timmermans Jean-Pierre
• Co-promoter: Adriaensen Dirk

Research team(s)

Project type(s)

• Research Project

Abstract

1) Enteric neurontypes are morphologically identified after intracellular marking; their synaptic input under normal circumstances is quantified; 2) They are morphologically characterized with inflammatory gut diseases and experimentally induced parasitory inflammation processes in the gut wall; 3) Study of the extracellular matrix and its role in cell migrations; 4) Postnatal development of the pulmonal acinus.

Researcher(s)

• Promoter: Timmermans Jean-Pierre
• Co-promoter: Harrisson Fernand
• Co-promoter: Van Meir Frans

Research team(s)

Project type(s)

• Research Project

Abstract

This project comprised the study of the neuroendocrine regulation mechanisms of visceral function with special attention to: 1. the non-adrenergic, non-cholinergic (NANC) component of the enteric nervous system; 2. the working mechanism of motilin; and 3. the relation between motility and inflammation.

Researcher(s)

• Promoter: Timmermans Jean-Pierre

Research team(s)

Project type(s)

• Research Project

Abstract

Using immunocytochemical and tracing techniques the topographical and immunocytochemical characterization of afferent nervepathways in relation to brain-gut interactions is attempted.

Researcher(s)

• Promoter: Scheuermann Dietrich
• Co-promoter: Timmermans Jean-Pierre

Research team(s)

Project type(s)

• Research Project