Blocking abundant RNA transcripts by high-affinity oligonucleotides during transcriptome library preparation

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Biological procedures online - ISSN 1480-9222-25:1 (2023) p. 1-19
Auteur(s)
    Celine Everaert, Jasper Verwilt, Kimberly Verniers, Niels Vandamme, Alvaro Marcos Rubio, Jo Vandesompele, Pieter Mestdagh

Artifacts and biases of the reverse transcription reaction in RNA sequencing

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RNA - ISSN 1355-8382-29:7 (2023) p. 889-897
Auteur(s)

Evaluation of efficiency and sensitivity of 1D and 2D sample pooling strategies for SARS-CoV-2 RT-qPCR screening purposes

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Scientific reports - ISSN 2045-2322-12:1 (2022) p. 1-10
Auteur(s)
    Jasper Verwilt, Jan Hellemans, Tom Sante, Pieter Mestdagh, Jo Vandesompele

The dangers of using Cq to quantify nucleic acid in biological samples : a lesson from COVID-19

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Clinical chemistry : international journal of laboratory medicine and molecular diagnostics - ISSN 0009-9147-68:1 (2022) p. 153-162
Auteur(s)
    Daniel Evans, Simon Cowen, Martin Kammel, Denise M. O’Sullivan, Graham Stewart, Hans-Peter Grunert, Jacob Moran-Gilad, Jasper Verwilt, Jiwon In, Jo Vandesompele, Kathryn Harris, Ki Ho Hong, Nathaniel Storey, Suzie Hingley-Wilson, Ulf Dühring, Young-Kyung Bae, Carole A. Foy, Julian Braybrook, Heinz Zeichhardt, Jim F. Huggett

When DNA gets in the way : a cautionary note for DNA contamination in extracellular RNA-seq studies

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Proceedings of the National Academy of Sciences of the United States of America - ISSN 0027-8424-117:32 (2020) p. 18934-18936
Auteur(s)
    Jasper Verwilt, Wim Trypsteen, Ruben Van Paemel, Katleen De Preter, Maria D. Giraldez, Pieter Mestdagh, Jo Vandesompele