Research team
Expertise
To determine electric interactions between cable bacteria and associated bacteria, light microscopy and more advanced microscopy types such as Electron- and Raman microscopy will be used combined with molecular techniques such as isolation of bacteria, DNA and RNA followed by next-generation sequencing and bioinformatics analyses of this data to determine presence of electroactivity and proteins/genes associated with extra-cellular electron exchange. Cable bacteria and their environment will be manipulated during these experiments on microscopy-sized microcosms. Sampling trips for sediment collection and determination of presence/absence of cable bacteria and electroactivity will be determined through microsensor profiling for O2, pH, EP and H2S (both in the field and in the lab).
Electric natural entryways (ENTER).
Abstract
The reach of biological electron transport (ET) increased from nm to cm with the discovery of cable bacteria that do ET via highly conductive fibres along their filaments. Their extremely long distance ET electrically connects 1000s of cells and influences redox cycling. Other bacteria interact with this electric highway via interspecies ET. A visual version: flocking, where aerobes use cables to breathe oxygen in its absence. Flockers dump electrons on intermediates, electron shuttles, which cables recycle. Since their discovery, cable bacteria sparked interest for green, biodegradable electronics. Flocking suggests that we can access the electric fibre without damaging it. Cables must have a natural electric entryway, to upload electrons from shuttles onto the fibres. ENTER aims to map this. We combine Prof Meysman's expertise on cable bacteria fibres with mine on flocker-cable bacteria interactions to: 1) Identify the electron shuttle by extensive electrochemical characterization of flockers (isolated in my PhD), map their ability to generate electricity, and find shuttle production potential in the genomes. 2) Advance models to find entryway protein sequences in closed cable bacteria genomes (from the host). 3) Localize the entryways on the filament and activate them using correlative light and electron microscopy with labelled shuttles and Raman microscopy. ENTER will provide new insights into the functioning of electric ecosystems and electric microbes. It will offer novel perspectives on redox and electron flow in natural systems. For example, oxygen breathing way beyond its presence will affect CO2 burying and sequestration in the seafloor. ENTERs impact, not limited to natural systems, will also inspire new insights for engineered systems (microbial fuel cells, contaminant biodegradation). It could provide critical stepping stones for promising alternatives in new green electronics.Researcher(s)
- Promoter: Meysman Filip
- Fellow: Lustermans Jamie
Research team(s)
Project type(s)
- Research Project